Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
Faecal pollution source tracking in the holy Bagmati River by portable 16S rRNA gene sequencing
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DOI：
10.1038/s41545-021-00099-1
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发表时间：
2021-02-18
期刊：
影响因子：
11.4
通讯作者：
Werner, David
中科院分区：
文献类型：
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作者：
Pantha, Kalyan;Acharya, Kishor;Werner, David
手提箱实验室用于16 S rRNA扩增子测序，以评估尼泊尔加德满都圣巴格马蒂河的微生物水质。SourceTracker分析和火山图显示，河流下游部分的微生物群落主要来自未经处理的污水。污水微生物组的季节性变化反映在下游河流水质中。在上游站点中，细菌属Acidovorax、Geobacillus和柄杆菌属占主导地位，而含有假定的人类病原体和肠道细菌的属，如梭菌属、普雷沃氏菌属、弓形杆菌属、乳杆菌属、肠球菌属和链球菌属在下游站点中变得突出。总细菌、总大肠菌群、人大肠杆菌的标记基因qPCR测定。coli、Arcobacter butzleri和Vibrio cholesterol证实了测序数据趋势。尽管基本的卫生设施现在在尼泊尔几乎是普遍的，但我们的研究结果表明，不适当的废水管理可能会使城市河流变成开放的下水道，从而构成公共卫生风险。
A suitcase laboratory was used for 16S rRNA amplicon sequencing to assess microbial water quality in the holy Bagmati River, Kathmandu, Nepal. SourceTracker analysis and Volcano plots revealed that microbial communities in the downstream part of the river were mainly contributed by untreated sewage. Seasonal variability in the sewage microbiome was reflected in the downstream river water quality. The bacterial genera Acidovorax, Geobacillus and Caulobacter predominated in the upstream sites, while genera containing putative human pathogens and gut bacteria, such as Clostridium, Prevotella, Arcobacter, Lactobacillus, Enterococcus and Streptococcus become prominent in the downstream sites. Marker gene qPCR assays for total bacteria, total coliforms, Human E. coli, Arcobacter butzleri and Vibrio cholerae confirmed the sequencing data trends. Even though basic sanitation provision is nowadays near universal in Nepal, our findings show how inadequate wastewater management may turn an urban river into an open sewer, which poses a public health risk.