Dietary betaine modifies hepatic metabolism but not renal injury in rat polycystic kidney disease
Dietary betaine modifies hepatic metabolism but not renal injury in rat polycystic kidney disease
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DOI：
10.1152/ajpgi.2000.279.6.g1162
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发表时间：
2000-12-01
影响因子：
4.5
通讯作者：
Peeling, J
中科院分区：
文献类型：
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作者：
Ogborn, MR;Nitschmann, E;Peeling, J
我们进行了形态测量和质子核磁共振（H-1-NMR）的研究，以测试的假设，即1%的饮食甜菜碱补充剂将改善肾脏疾病的杂合子Han：SPRD-cy大鼠，多囊肾病（PKD）和进行性慢性肾功能衰竭的模型。配对喂养8周后，甜菜碱对肾脏囊性变、肾间质纤维化、血清肌酐、血清胆固醇或血清甘油三酯没有影响。肾组织的H-1-NMR光谱显示，肾脏渗透压，包括甜菜碱，或其他有机阴离子的肾脏含量在响应饮食没有变化。为探索摄入甜菜碱的代谢命运而进行的肝组织的H-1-NMR光谱显示，与没有肾病的动物相比，喂食对照饮食的杂合动物具有升高的肝致坏死氨基酸水平、升高的β-羟基丁酸盐水平和升高的一些柠檬酸循环代谢物水平。补充甜菜碱消除了这些变化。汉族慢性肾衰竭：SPRD-cy大鼠与肝代谢紊乱相关，可通过甜菜碱治疗纠正，表明这种形式的慢性肾衰竭中存在可逆的甲基化缺陷。
We undertook a morphometric and proton nuclear magnetic resonance (H-1-NMR) study to test the hypothesis that 1% dietary betaine supplementation would ameliorate renal disease in the heterozygous Han:SPRD-cy rat, a model of polycystic kidney disease (PKD) and progressive chronic renal failure. After 8 wk of pair feeding, betaine had no effect on renal cystic change, renal interstitial fibrosis, serum creatinine, serum cholesterol, or serum triglycerides. H-1-NMR spectroscopy of renal tissue revealed no change in renal osmolytes, including betaine, or renal content of other organic anions in response to diet. H-1-NMR spectroscopy of hepatic tissue performed to explore the metabolic fate of ingested betaine revealed that heterozygous animals fed the control diet had elevated hepatic levels of gluconeogenic amino acids, increased beta -hydroxybutyrate, and increased levels of some citric acid cycle metabolites compared with animals without renal disease. Betaine supplementation eliminated these changes. Chronic renal failure in the Han:SPRD-cy rat is associated with disturbances of hepatic metabolism that can be corrected with betaine therapy, suggesting the presence of a reversible methylation defect in this form of chronic renal failure.