Morphological and pharmacological characterization of the porcine popliteal artery: A novel model for study of lower limb arterial disease.
Morphological and pharmacological characterization of the porcine popliteal artery: A novel model for study of lower limb arterial disease.
复制标题
猪腘动脉的形态学和药理学特征：研究下肢动脉疾病的新模型。
DOI：
10.1111/micc.12527
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Bagher,Pooneh
中科院分区：
文献类型：
--
作者：
Frederick,NormanE;Mitchell,Ray;Hein,TravisW;Bagher,Pooneh
本研究旨在表征猪腘动脉的结构和药理学特性，以开发一种用于检查各种心血管疾病中下肢血流调节的新系统，方法从链脲佐菌素诱导的糖尿病猪或年龄匹配的盐水注射对照猪中分离腘动脉，使用透射电子显微镜进行形态学研究结果猪腘动脉壁的透射电子显微镜显示存在内皮细胞-平滑肌细胞相互作用（肌内皮连接）和平滑肌细胞-平滑肌细胞相互作用，我们为此创造了术语“肌-肌连接”。这些肌-肌连接显示出指示连接蛋白表达的斑块。此外，猪腘动脉对各种血管收缩剂（包括去甲肾上腺素、苯肾上腺素和U46619）和血管扩张剂（包括乙酰胆碱、腺苷5′-[β-硫代]二磷酸和缓激肽）具有高度反应性。最后，2周后链脲佐菌素诱导的糖尿病，去甲肾上腺素的猪腘动脉的正常化血管收缩是不变的control.ConclusionsThe猪腘动脉显示结构和药理学特性，可能证明在未来的研究中有用的糖尿病相关的外周动脉疾病和其他下肢心血管疾病。
ObjectiveThis study was undertaken to characterize structural and pharmacological properties of the pig popliteal artery in order to develop a novel system for the examination of lower limb blood flow regulation in a variety of cardiovascular pathologies, such as diabetes‐induced peripheral artery disease.MethodsPopliteal arteries were isolated from streptozocin‐induced diabetic pigs or age‐matched saline‐injected control pigs for morphological study using transmission electron microscopy and for examination of vasoreactivity to pharmacological agents using wire myography.ResultsTransmission electron microscopy of the porcine popliteal artery wall revealed the presence of endothelial cell‐smooth muscle cell interactions (myoendothelial junctions) and smooth muscle cell‐smooth muscle cell interactions, for which we have coined the term “myo‐myo junctions.” These myo‐myo junctions were shown to feature plaques indicative of connexin expression. Further, the pig popliteal artery was highly responsive to a variety of vasoconstrictors including norepinephrine, phenylephrine, and U46619, and vasodilators including acetylcholine, adenosine 5′‐[β‐thio] diphosphate, and bradykinin. Finally, 2 weeks after streptozocin‐induced diabetes, the normalized vasoconstriction of the pig popliteal artery to norepinephrine was unaltered compared to control.ConclusionsThe pig popliteal artery displays structural and pharmacological properties that might prove useful in future studies of diabetes‐associated peripheral artery disease and other lower limb cardiovascular diseases.