Spatiotemporal distribution patterns of oligosaccharides during early embryogenesis in the starfish Patiria pectinifera
Spatiotemporal distribution patterns of oligosaccharides during early embryogenesis in the starfish Patiria pectinifera
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DOI：
10.1007/s00427-009-0280-1
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发表时间：
2009-04-01
影响因子：
2.4
通讯作者：
Matsuda, Seiji
中科院分区：
文献类型：
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作者：
Doihara, Takuya;Miguchi, Yuji;Matsuda, Seiji
为了研究海星Patiria（Asterina）pectinifera的胚胎发生机制，我们组织化学分析了几个幼虫阶段使用阿尔新蓝（AB，染色酸性粘蛋白），高碘酸希夫（PAS，染色中性粘蛋白），和21种类型的凝集素。糖在细胞质、基膜和囊胚腔中的分布模式如下：（1）第一组凝集素在中内胚层细胞中显示颗粒状信号，这些凝集素可能是有用的中内胚层标记物。(2)第二类凝集素从孵化的囊胚到中期原肠胚的整个细胞质中显示出弥散信号。这些信号成为本地化的原肠细胞的基底细胞质在bipinnaria早期。(3)凝集素反应在基膜达到高峰的早期至中期原肠胚和几乎消失的早期bipinnaria。这些结果表明，存在的各种物质的基底膜，并暗示这些物质的重要性，在原肠的延伸和间充质分化的诱导。(4)在这些阶段中，囊胚腔中AB-PAS双重染色的信号颜色从洋红（PAS染色）变为蓝色（AB染色），因此，表明位于囊胚腔中的粘蛋白从中性变为酸性。本报告最重要的部分是第一次描述了许多不同的凝集素和染色剂的组合的内和细胞外成分的特性的时间变化。
To examine embryogenic mechanisms in the starfish Patiria (Asterina) pectinifera, we histochemically analyzed several larval stages using Alcian Blue (AB, which stains acidic mucins), Periodic Acid Schiff (PAS, which stains neutral mucins), and 21 types of lectins. Carbohydrate distribution patterns were observed in the cytoplasm, basement membrane, and blastocoel as follows: (1) The first group of lectins showed granular signals in the mesendodermal cells, and these lectins may be useful as mesendoderm markers. (2) The second class of lectins showed diffuse signals across the entire cytoplasm from the hatched blastula until the mid gastrula. These signals became localized to the basal cytoplasm of archenteron cells at the early bipinnaria. (3) Lectin reactivity in the basement membrane peaked at the early-to-mid gastrula and was nearly gone by the early bipinnaria. These results suggest the existence of various substances in the basement membrane and imply the importance of these substances during archenteron elongation and the induction of mesenchyme differentiation. (4) Signal colors with AB-PAS double staining in the blastocoel changed from magenta (by PAS staining) into blue (by AB staining) during these stages, thus, indicating that mucin located in the blastocoel changed from neutral to acidic. The most significant part of this report is the first description regarding temporal changes in the characteristics of intra- and extracellular components with the combination of many different lectins and stains.