RNA-dependent RNA polymerase 6 of rice (Oryza sativa) plays role in host defense against negative-strand RNA virus, Rice stripe virus
RNA-dependent RNA polymerase 6 of rice (Oryza sativa) plays role in host defense against negative-strand RNA virus, Rice stripe virus
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水稻 (Oryza sativa) 的 RNA 依赖性 RNA 聚合酶 6 在宿主防御负链 RNA 病毒（水稻条纹病毒）中发挥作用。
DOI：
10.1016/j.virusres.2011.11.016
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发表时间：
2012-02-01
期刊：
影响因子：
5
通讯作者：
Li, Yi
中科院分区：
文献类型：
--
作者：
Jiang, Lin;Qian, Dan;Li, Yi
来自真菌、植物和一些无脊椎动物的RNA依赖性RNA聚合酶（RDRs）在抗病毒防御中起着重要作用。在这里，我们研究了RDR 6在防御经济上重要的水稻植物对负链RNA病毒（水稻条纹病毒，RSV），造成巨大的作物损害的作用。在三个独立的转基因株系（OsRDREAS株系A、B和C）中，其中OsRDR 6的转录水平通过反义沉默降低了70-80%，显示RSV的感染和疾病症状显著增强。OsRDR 6AS植物的过敏性不归因于增强的昆虫侵染，而是归因于增强的病毒感染。症状的增加与0 sRDR 6AS植物中RSV基因组RNA的积累增加相关。深度测序数据显示，与野生型植物相比，0 sRDR 6AS植物中RSV来源的siRNA积累减少。这是第一份关于RDR在单子叶作物植物中防御负链RNA病毒的抗病毒作用的报告，并显著扩展了RDR在许多植物群体中防御不同类型病毒基因组的抗病毒作用的现有知识。(C)2011爱思唯尔有限公司版权所有。
RNA-dependent RNA polymerases (RDRs) from fungi, plants and some invertebrate animals play fundamental roles in antiviral defense. Here, we investigated the role of RDR6 in the defense of economically important rice plants against a negative-strand RNA virus (Rice stripe virus, RSV) that causes enormous crop damage. In three independent transgenic lines (OsRDREAS line A, B and C) in which OsRDR6 transcription levels were reduced by 70-80% through antisense silencing, the infection and disease symptoms of RSV were shown to be significantly enhanced. The hypersusceptibilities of the OsRDR6AS plants were attributed not to enhanced insect infestation but to enhanced virus infection. The rise in symptoms was associated with the increased accumulation of RSV genomic RNA in the OsRDR6AS plants. The deep sequencing data showed reduced RSV-derived siRNA.accumulation in the OsRDR6AS plants compared with the wild type plants. This is the first report of the antiviral role of a RDR in a monocot crop plant in the defense against a negative-strand RNA virus and significantly expands upon the current knowledge of the antiviral roles of RDRs in the defense against different types of viral genomes in numerous groups of plants. (C) 2011 Elsevier B.V. All rights reserved.