Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association
Promoter-specific binding of Rap1 revealed by genome-wide maps of protein-DNA association
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DOI：
10.1038/ng569
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发表时间：
2001-08-01
期刊：
影响因子：
30.8
通讯作者：
Brown, PO
中科院分区：
文献类型：
--
作者：
Lieb, JD;Liu, XL;Brown, PO
我们确定了分布的阻遏物激活蛋白1（Rap1）和辅助沉默蛋白Sir2，Sir3和Sir4在体内的整个酵母基因组，在2 kb的分辨率。Rap1在快速生长期间对细胞经济至关重要，靶向294个位点，约5%的酵母基因，并参与指数生长细胞中所有RNA聚合酶II起始事件的37%的激活。尽管Rap1识别的DNA序列存在于编码序列和基因间序列中，但Rap1与基因组的结合对基因间区域具有高度特异性，具有作为启动子的潜力。这种全局现象可能是序列特异性转录因子的普遍特征，表明存在一种标记启动子区域的全基因组分子机制。
We determined the distribution of repressor-activator protein 1 (Rap1) and the accessory silencing proteins Sir2, Sir3 and Sir4 in vivo on the entire yeast genome, at a resolution of 2 kb. Rap1 is central to the cellular economy during rapid growth, targeting 294 loci, about 5% of yeast genes, and participating in the activation of 37% of all RNA polymerase II initiation events in exponentially growing cells. Although the DNA sequence recognized by Rap1 is found in both coding and intergenic sequences, the binding of Rap1 to the genome was highly specific to intergenic regions with the potential to act as promoters. This global phenomenon, which may be a general characteristic of sequence-specific transcriptional factors, indicates the existence of a genome-wide molecular mechanism for marking promoter regions.