Isolation of dendritic cells.
Isolation of dendritic cells.
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DOI：
10.1002/0471142735.im0307s86
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发表时间：
2009-08-01
影响因子：
--
通讯作者：
Brinster, Carine
中科院分区：
文献类型：
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作者：
Inaba, Kayo;Swiggard, William J;Brinster, Carine
本单元介绍了两种制备树突状细胞（DC）的方法，树突状细胞是一种高度特化的抗原呈递细胞（APC）。第一种方法涉及从小鼠脾脏中分离DC，产生高度富集辅助细胞和APC功能的细胞群。胶原酶消化脾细胞悬液的支持协议，以增加树突状细胞的产量。第二种方法涉及从小鼠骨髓祖细胞产生大量DC。在该技术中，骨髓细胞在粒细胞/巨噬细胞集落刺激因子（GM-CSF）的存在下培养以产生5-10 × 10（6）个细胞，其中60%表达DC表面标志物（例如，B-7-2/CD86）。还讨论了从小鼠脾或其他小鼠组织以及从人组织分离DC的其他技术。
This unit presents two methods for preparing dendritic cells (DCs), a highly specialized type of antigen-presenting cell (APC). The first method involves the isolation of DCs from mouse spleen, resulting in a cell population that is highly enriched in accessory cell and APC function. A support protocol for collagenase digestion of splenocyte suspensions is described to increase the yield of dendritic cells. The second method involves generating large numbers of DCs from mouse bone marrow progenitor cells. In that technique, bone marrow cells are cultured in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF) to yield 5-10 x 10(6) cells, 60% of which express DC surface markers (e.g., B-7-2/CD86). Additional techniques for isolating DCs from mouse spleens or other mouse tissues, as well as from human tissues, are also discussed.