Plasma Concentrations of High-Mobility Group Box Protein 1, Soluble Receptor for Advanced Glycation End-Products and Circulating DNA in Patients with Acute Pancreatitis
Plasma Concentrations of High-Mobility Group Box Protein 1, Soluble Receptor for Advanced Glycation End-Products and Circulating DNA in Patients with Acute Pancreatitis
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DOI：
10.1159/000181172
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发表时间：
2009-01-01
期刊：
影响因子：
3.6
通讯作者：
Mandi, Y.
中科院分区：
文献类型：
--
作者：
Kocsis, A. K.;Szabolcs, A.;Mandi, Y.
目的：高迁移率族蛋白1（HMGB 1）是一种迟发性促炎细胞因子，由炎症细胞主动分泌，并从坏死细胞被动释放。从炎症和坏死共同参与急性胰腺炎发病机制的角度出发，本研究旨在联合检测急性胰腺炎患者血浆中HMGB 1、其可溶性糖基化终产物受体（soluble receptor for advanced glycation end-products，sNAs）和作为细胞死亡标志物的循环DNA的浓度。方法：选择急性胰腺炎患者62例（轻型30例，重型32例），脓毒症患者20例，健康对照者20例。采用ELISA法测定血浆HMGB 1和血清TNF-α水平。通过β-珠蛋白基因的实时定量PCR估计血浆DNA浓度。结果如下：重症急性胰腺炎患者的循环HMGB 1水平（13.33 +/- 2.11 ng/ml）显著高于健康对照组（0.161 +/- 0.03 ng/ml）或轻度胰腺炎患者（2.64 +/- 0.185 ng/ml）。脓毒症患者血浆sIgA浓度最高（2，210 +/- 252 pg/ml），而急性胰腺炎患者sIgA水平与HMGB 1水平呈负相关。血浆DNA水平在重症急性胰腺炎患者中显著升高（2，206 +/- 452 ng/ml）。结论：对血浆HMGB 1、sIgA和循环DNA水平的复杂研究可为评估不同严重程度的急性胰腺炎提供信息。版权所有（C）2009 S. Karger AG、巴塞尔和IAP
Aims: High-mobility group box protein 1 (HMGB1), a late-acting proinflammatory cytokine, is secreted actively by inflammatory cells, and released passively from necrotic cells. From the aspect that both inflammation and necrosis are involved in the pathogenesis in acute pancreatitis, the aim of the study was a joint investigation of the plasma concentrations of HMGB1, its soluble receptor for advanced glycation end-products (sRAGE), and the circulating DNA as a marker of cell death. Methods: 62 patients with acute pancreatitis (30 mild, 32 severe), 20 patients with sepsis, and 20 healthy controls were enrolled in the study. HMGB1 and sRAGE plasma levels were measured by means of ELISA. Plasma DNA concentrations were estimated by real-time quantitative PCR for the beta-globin gene. Results: The circulating HMGB1 level was significantly higher in patients with severe acute pancreatitis (13.33 +/- 2.11 ng/ml) than in healthy controls (0.161 +/- 0.03 ng/ml) or than in patients with mild pancreatitis (2.64 +/- 0.185 ng/ml). The plasma concentration of sRAGE was highest in patients with sepsis (2,210 +/- 252 pg/ml), while the levels of sRAGE correlated inversely with that of HMGB1 in patients with acute pancreatitis. The plasma DNA level was significantly elevated in patients with severe acute pancreatitis (2,206 +/- 452 ng/ml). Conclusion: A complex study of the plasma levels of HMGB1, sRAGE and circulating DNA can be informative in evaluations of acute pancreatitis with different levels of severity. Copyright (C) 2009 S. Karger AG, Basel and IAP