TIME-DEPENDENT RECOVERY OF PASSIVE NEUTROPHILS AFTER LARGE DEFORMATION
TIME-DEPENDENT RECOVERY OF PASSIVE NEUTROPHILS AFTER LARGE DEFORMATION
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DOI：
10.1016/s0006-3495(91)82119-1
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发表时间：
1991-10-01
影响因子：
3.4
通讯作者：
HOCHMUTH, RM
中科院分区：
文献类型：
--
作者：
TRANSONTAY, R;NEEDHAM, D;HOCHMUTH, RM
进行实验，其中通过将被动的人嗜中性粒细胞吸入小玻璃移液管中使其变形为细长的“香肠”形状。 当从移液管中排出时，中性粒细胞在约1分钟内恢复其天然球形。 这个回收过程进行了分析，根据一个牛顿，液滴模型，其中的变分法是用来同时解决流体动力学方程低雷诺数流和膜平衡方程与恒定的膜张力。 理论模型给出了一个很好的拟合膜皮质张力细胞质粘度的比值约为1.7 × 10（-5）cm/s（0.17 μ m/s）的实验数据。 然而，当细胞在移液管中仅保持5秒或更短的时间段，然后排出时，细胞经历初始的快速弹性回弹，表明细胞在这种情况下表现为麦克斯韦粘弹性液体，而不是具有恒定皮质张力的牛顿液体。
Experiments are performed in which a passive human neutrophil is deformed into an elongated "sausage" shape by aspirating it into a small glass pipette. When expelled from the pipette the neutrophil recovers its natural spherical shape in approximately 1 minute. This recovery process is analyzed according to a Newtonian, liquid-drop model in which a variational method is used to simultaneously solve the hydrodynamic equations for low Reynolds-number flow and the equations for membrane equilibrium with a constant membrane tension. The theoretical model gives a good fit to the experimental data for a ratio of membrane cortical tension to cytoplasmic viscosity of approximately 1.7 x 10(-5) cm/s (0.17-mu-m/s). However, when the cell is held in the pipette for only a short time period of 5 s or less, and then expelled, the cell undergoes an initial, rapid elastic rebound suggesting that the cell behaves in this instance as a Maxwell viscoelastic liquid rather than a Newtonian liquid with constant cortical tension.