Fungal degradation of poly(ethylene succinate)
Fungal degradation of poly(ethylene succinate)
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DOI：
10.1016/j.polymdegradstab.2006.09.014
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发表时间：
2007-01-01
影响因子：
5.9
通讯作者：
Kasuya, Ken-ichi
中科院分区：
文献类型：
--
作者：
Ishii, Nariaki;Inoue, Yoshio;Kasuya, Ken-ichi
从不同的环境样品中分离出20种真菌，它们在含有聚琥珀酸乙烯（PESu）的培养基上围绕菌落形成一个清晰的区域。中温菌株NKCM1003在含PESu为唯一碳源的培养基上，30℃好氧培养，降解PESu膜的速率为21 +/- 2 μ g/cm(2)/h，具有最高的PESu水解活性。SEM观察表明，应变从表面的非晶态区开始逐渐降解薄膜。系统发育分析表明该菌株与曲霉（Aspergillus clavatus）亲缘关系较近。酶谱分析表明分泌的酶具有PESu水解活性，为P（3HB）解聚合酶。该菌株还利用了PESu的酶产物，使其生长良好。这些结果表明，菌株NKCM1003在田间pesu降解过程中发挥了重要作用。(c) 2006 Elsevier Ltd.版权所有。
Twenty fungi, which all formed a clear zone around the colony on a poly(ethylene succinate) (PESu)-containing medium, were isolated from various environmental samples. Mesophilic strain NKCM1003, with the highest PESu hydrolytic activity among all the isolates, degraded a PESu film at the rate of 21 +/- 2 mu g/cm(2)/h when it was aerobically incubated at 30 degrees C on a medium containing PESu as the sole carbon source. SEM observations showed that the strain gradually degraded the film starting from the amorphous regions of the surface. Phylogenetic analysis revealed that the strain was closely related to the species Aspergillus clavatus. Zymogram analysis suggested that the secreted enzyme with PESu hydrolytic activity is a P(3HB) depolymerase. The strain also utilized the enzymatic products of PESu, permitting it to grow well. These results indicate that the strain NKCM1003 plays an important role in the PESu-degrading process in the field. (c) 2006 Elsevier Ltd. All rights reserved.