The Relative Errors of Bacteriological Plate Counting Methods
The Relative Errors of Bacteriological Plate Counting Methods
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细菌平板计数方法的相对误差
DOI：
10.1128/jb.54.5.641-654.1947
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发表时间：
1947
影响因子：
3.2
通讯作者：
T. Snyder
中科院分区：
文献类型：
--
作者：
T. Snyder
方法采用三种测定接种物的方法，并结合其他技术改进，提供了五种平板接种方法进行比较：A.将制备和干燥的平板接种6滴通过单个校准毛细管移液器递送的微生物稀释液。允许液滴落在琼脂表面上的良好分离的点上。(1)通过在所有方向上重复倾斜板来增加由单个液滴覆盖的面积。该方法通常用于布鲁氏菌属。
METHODSThree methods of measuring aliquots of inoculum were employed, and these, together with other modifications of technique, provided five plating methods for comparison, as follows:A. Prepared and dried plateswere inoculated with 6 drops of a klown dilution of organisms delivered by means of a single calibrated capillary pipette.-The drops were allowedto fall at well-separated points on the surface of the agar.(1) The area covered by the individual drops was increased by repeatedly tilting the plate in all directions. This method is commonly used with Brucella species.