Hepatoma-derived growth factor is a novel prognostic factor for hepatocellular carcinoma
Hepatoma-derived growth factor is a novel prognostic factor for hepatocellular carcinoma
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DOI：
10.1245/aso.2006.11.035
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发表时间：
2006-02-01
影响因子：
3.7
通讯作者：
Nakamura, H
中科院分区：
文献类型：
--
作者：
Yoshida, K;Tomita, Y;Nakamura, H
背景：肝癌源性生长因子（HDGF）参与肝癌的发生，以及肝脏的发育和再生。本研究探讨HDGF表达与肝细胞癌（HCC）分化及预后的关系。方法：采用免疫组化方法分析100例接受手术治疗的肝癌患者（男性81例，女性19例）的HDGF表达情况，年龄34 ~ 81岁（中位61岁）。逆转录聚合酶链反应检测10例HDGF信使RNA表达。hcc的免疫染色模式分为HDGF指数阳性（细胞核和细胞质中90%以上的肿瘤细胞均呈阳性染色）或HDGF指数阴性（其余均为阴性）。结果：HDGF指数阳性27例（27%），阴性73例（73%）。HDGF指数阳性的4例患者的HDGF信使RNA表达明显高于指数阴性的6例患者。组织学特征高分化的患者HDGF指数阳性率高于低分化亚型患者。单因素和多因素分析显示，与指数为阴性的患者相比，HDGF指数阳性的患者的无病生存率和总生存率明显较差。结论：这些发现提示HDGF免疫组化在确定HCC预后方面的潜在应用。
Background: Hepatoma-derived growth factor (HDGF) is involved in hepatocarcinogenesis, as well as in liver development and regeneration. This study investigated the correlation of HDGF expression with differentiation and prognosis of hepatocellular carcinoma (HCC).Methods: HDGF expression in 100 patients with HCC (81 men and 19 women) with ages ranging from 34 to 81 years (median, 61 years) receiving surgical treatment was analyzed by immunohistochemistry. HDGF messenger RNA expression was evaluated in 10 cases by reverse transcription-polymerase chain reaction. The immunostaining pattern in HCCs was categorized as a positive HDGF index (showing positive staining in > 90% of tumor cells in both nucleus and cytoplasm) or a negative HDGF index (all others).Results: Twenty-seven cases (27%) showed a positive and 73 (73%) showed a negative HDGF index. HDGF messenger RNA expression was significantly higher in four cases with a positive HDGF index than in six with a negative index. Cases with well-differentiated histological characteristics showed a higher rate of positive HDGF index than those with a poorly differentiated subtype. Univariate and multivariate analysis revealed significantly poorer disease-free and overall survivals in patients with a positive HDGF index compared with patients with a negative index.Conclusion: These findings suggest the potential utility of HDGF immunohistochemistry in determining the prognosis of HCC.