High prevalence of CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand
High prevalence of CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand
复制标题
DOI：
10.1093/jac/dkq008
复制
发表时间：
2010-04-01
影响因子：
5.2
通讯作者：
Yamamoto, Yoshimasa
中科院分区：
文献类型：
--
作者：
Sasaki, Tadahiro;Hirai, Itaru;Yamamoto, Yoshimasa
目的 确定泰国农村地区健康个体粪便标本中产 CTX-M β-内酰胺酶肠杆菌科细菌的患病率。在含有头孢噻肟的麦康凯琼脂上筛查粪便标本中的细菌是否产生超广谱 β-内酰胺酶 (ESBL)，并通过双盘协同试验进行确认。使用从分离株中提取的细菌 DNA，通过 PCR 进行 CTX-M 型 ESBL 的基因检测和基因分型。表型和基因检查均证实，大量标本（141 份中的 82 份，58.2%）显示存在产生 CTX-M β-内酰胺酶的肠杆菌科细菌。大多数产生CTX-M β-内酰胺酶的细菌是大肠杆菌（85.1%）。研究表明，产生CTX-M β-内酰胺酶的肠杆菌科细菌在健康人群中广泛传播。
To determine the prevalence of CTX-M beta-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in a rural area of Thailand.Bacteria in stool specimens were screened for extended-spectrum beta-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test. Genetic detection and genotyping of CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates.A markedly high number (82 of 141, 58.2%) of the specimens showed the presence of CTX-M beta-lactamase-producing Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority of the CTX-M beta-lactamase-producing bacteria were Escherichia coli (85.1%).The study revealed the wide dissemination of CTX-M beta-lactamase-producing Enterobacteriaceae in the healthy population.