Identification and characterization of a novel cytotoxic protein, parasporin-4, produced by Bacillus thuringiensis A1470 strain
Identification and characterization of a novel cytotoxic protein, parasporin-4, produced by Bacillus thuringiensis A1470 strain
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DOI：
10.1016/s1387-2656(08)00009-4
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Inouye, Kuniyo
中科院分区：
文献类型：
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作者：
Okumura, Shiro;Saitoh, Hiroyuki;Inouye, Kuniyo
1901年，一种独特的细菌被分离出来作为蚕蛾幼虫的sotto病的病原体，后来在1915年，这种生物被描述为苏云金芽孢杆菌。自发现以来，这种细菌不仅引起了昆虫病理学家的广泛关注，而且引起了许多其他科学家的关注，这些科学家对与B的包涵体相关的强且特异的杀虫活性感兴趣。苏云金杆菌这导致了最近世界范围内B的发展。苏云金杆菌为基础的微生物杀虫剂和抗虫转基因植物，以及划时代的发现parasporin，癌细胞特异性细胞毒素。本文介绍了B包涵体蛋白相互作用的检测方法。利用表面等离子体共振生物传感器对苏云金杆菌和昆虫刷状缘细胞膜进行了初步研究，并对B产生的一种新的癌细胞杀伤蛋白parasporin-4进行了鉴定和克隆。苏云金杆菌A1470菌株。用一种新的方法对重组大肠杆菌产生的副孢蛋白4包涵体进行了溶解和活化，并通过阴离子交换层析进行了纯化。最后对重组副孢子蛋白-4进行了鉴定。
In 1901, a unique bacterium was isolated as a pathogen of the sotto disease of the silkmoth larvae, and later in 1915, the organism was described as Bacillus thuringiensis. Since the discovery, this bacterium has widely attracted attention of not only insect pathologists but many other scientists who are interested in strong and specific insecticidal activity associated with inclusion bodies of B. thuringiensis. This has led to the recent worldwide development of B. thuringiensis-based microbial insecticides and insect-resistant transgenic plants, as well as the epoch-making discovery of parasporin, a cancer cell-specific cytotoxin. In the review, we introduce a detection study of interaction between inclusion proteins of B. thuringiensis and brush border membrane of insects using surface plasmon resonance-based biosensor, and then identification and cloning of parasporin-4, a latest cancer cell-killing protein produced by B. thuringiensis A1470 strain. Inclusion bodies of the parasporin-4 produced by recombinant Escherichia coli were solubilized and activated with a new method and purified by an anion-exchange chromatography. At last the characterization of the recombinant parasporin-4 was shown.