Virus-induced gene silencing in tomato fruit
Virus-induced gene silencing in tomato fruit
复制标题
番茄果实中病毒诱导的基因沉默。
DOI：
10.1111/j.1365-313x.2005.02441.x
复制
发表时间：
2005-07-01
期刊：
影响因子：
7.2
通讯作者：
Luo, YB
中科院分区：
文献类型：
--
作者：
Fu, DQ;Zhu, BZ;Luo, YB
病毒诱导基因沉默（VIGS）是研究植物基因功能的有力工具。在此，我们报告了通过注射器将烟草鼠疫病毒（TRV）载体注入附着在植株上的番茄果实的表面、茎或果实基部，或通过真空注入脱离植株的番茄果实，TRV 可在番茄果实中有效传播和复制。虽然可以通过上述所有方法在番茄果实中进行 VIGS，但最有效的方法是在授粉后 10 天左右将 TRV 病毒载体注入附着在植株上的幼果的果实基部。在果实发育过程中，包括 LeCTR1 和 LeElLs 基因在内的几个与乙烯反应和果实成熟相关的报告基因也被成功沉默。此外，我们还发现沉默 LeEIN2 基因会抑制番茄果实的成熟。我们的研究结果表明，通过所述方法应用 VIGS 技术可以成功地应用于番茄果实，是研究果实发育过程中相关基因功能的重要工具。
Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants. Here we report that either by syringe-infiltrating the tobacco rattle virus (TRV)-vector into the surface, stem or carpopodium of a tomato fruit attached to the plant or by vacuum-infiltrating into a tomato fruit detached from the plant, TRV can efficiently spread and replicate in the tomato fruit. Although VIGS can be performed in tomato fruit by all of the means mentioned above, the most effective method is to inject the TRV-vector into the carpopodium of young fruit attached to the plant about 10 days after pollination. Several reporter genes related to ethylene responses and fruit ripening, including LeCTR1 and LeEILs genes, were also successfully silenced by this method during fruit development. In addition, we found that the silencing of the LeEIN2 gene results in the suppression of tomato fruit ripening. The results of our study indicate that the application of VIGS techniques by the described methods can be successfully applied to tomato fruit and is a valuable tool for studying functions of the relevant genes during fruit developing.