Apoptosis Evaluation by Electrochemical Techniques.
Apoptosis Evaluation by Electrochemical Techniques.
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DOI：
10.1002/asia.201501045
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Jian Yin;Peng Miao
中科院分区：
文献类型：
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作者：
Jian Yin;Peng Miao
细胞凋亡与病理学、药理学和毒理学密切相关。准确、便捷的细胞凋亡检测将有助于生物学研究、临床诊断和药物开发。基于凋亡细胞的独特特征，多种分析技术被用于细胞凋亡的敏感分析，如表面等离子体共振、电化学方法、流式细胞术和一些成像分析。其中，电化学技术简单、易操作、成本低、灵敏度高的特点使其成为研究体外生物系统电子转移过程的有力工具。在这篇文章中，提供了当前关于细胞凋亡评估的各种技术方法的总体概述。综述了近年来用于细胞凋亡检测的电化学生物传感器及其相对于传统方法的优势。其中一个主要的考虑是设计基于细胞凋亡过程中各种生化事件的识别元素。
Apoptosis has close relevance to pathology, pharmacology, and toxicology. Accurate and convenient detection of apoptosis would be beneficial for biological study, clinical diagnosis, and drug development. Based on distinct features of apoptotic cells, a diversity of analytical techniques have been exploited for sensitive analysis of apoptosis, such as surface plasmon resonance, electrochemical methods, flow cytometry, and some imaging assays. Among them, the features of simplicity, easy operation, low cost, and high sensitivity make electrochemical techniques powerful tools to investigate electron-transfer processes of in vitro biological systems. In this contribution, a general overview of current knowledge on various technical approaches for apoptosis evaluation is provided. Furthermore, recently developed electrochemical biosensors for detecting apoptotic cells and their advantages over traditional methods are summarized. One of the main considerations focuses on designing the recognition elements based on various biochemical events during apoptosis.