Characterization of the human immune cell network at the gingival barrier.
Characterization of the human immune cell network at the gingival barrier.
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DOI：
10.1038/mi.2015.136
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发表时间：
2016-09
影响因子：
8
通讯作者：
Moutsopoulos NM
中科院分区：
文献类型：
--
作者：
Dutzan N;Konkel JE;Greenwell-Wild T;Moutsopoulos NM
口腔粘膜是一个屏障部位，经常暴露在丰富多样的共生微生物群落中，但对维持这一界面上的免疫动态平衡的免疫细胞网络知之甚少。我们在一大批健康受试者中对口腔免疫细胞亚群进行了详细的描述。我们将研究重点放在牙龈界面，这是一个特别脆弱的粘膜部位，具有薄薄的上皮衬里，并持续暴露在牙齿附着的生物膜中。在健康人群中，我们发现T细胞占主导地位，最小的B细胞，大量的粒细胞/中性粒细胞，专业抗原提呈细胞(APC)的复杂网络和少量的天然淋巴样细胞(ILC)监督牙龈屏障。我们进一步描述了健康人群中的细胞亚型，并询问了常见口腔炎症性牙周炎中免疫细胞群的变化。在疾病中，我们记录了中性粒细胞的增加和IL-17反应的上调。我们确定了健康和牙周炎患者中的主要IL-17来源为CD4+T细胞。总体而言，我们的研究提供了生理性口服免疫格局的第一个视角，并为局部免疫病理的表征提供了基线。
The oral mucosa is a barrier site constantly exposed to rich and diverse commensal microbial communities, yet little is known of the immune cell network maintaining immune homeostasis at this interface. We have performed a detailed characterization of the immune cell subsets of the oral cavity in a large cohort of healthy subjects. We focused our characterization on the gingival interface, a particularly vulnerable mucosal site, with thin epithelial lining and constant exposure to the tooth adherent biofilm. In health, we find a predominance of T cells, minimal B cells, a large presence of granulocytes/neutrophils, a sophisticated network of professional antigen presenting cells (APC) and a small population of innate lymphoid cells (ILC) policing the gingival barrier. We further characterize cellular subtypes in health and interrogate shifts in immune cell populations in the common oral inflammatory disease periodontitis. In disease we document an increase in neutrophils and an up-regulation of IL-17 responses. We identify the main source of IL-17 in health and periodontitis within the CD4+ T cell compartment. Collectively our studies provide a first view of the landscape of physiologic oral immunity and serve as a baseline for the characterization of local immunopathology.