High-frequency electrical stimulation reduced hyperalgesia and the activation of the Myd88 and NFκB pathways in chronic constriction injury of sciatic nerve-induced neuropathic pain mice
High-frequency electrical stimulation reduced hyperalgesia and the activation of the Myd88 and NFκB pathways in chronic constriction injury of sciatic nerve-induced neuropathic pain mice
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DOI：
10.1016/j.neulet.2023.137064
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发表时间：
2023-01
影响因子：
2.5
通讯作者：
Peixin Lu;Kexin Fang;Wen-Ling Cheng;Bin Yu
中科院分区：
文献类型：
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作者：
Peixin Lu;Kexin Fang;Wen-Ling Cheng;Bin Yu
神经性疼痛已经成为一个全球性的公共问题和健康负担。药物干预是主要的治疗方法，但药物治愈率低且有副作用。迫切需要开发新的治疗方法。高频电刺激（KHES）已广泛应用于临床镇痛。然而，人们对其机制知之甚少。在这项研究中，与神经性疼痛相关的数据集来自GEO数据库。通过功能富集分析对差异表达基因（DEGs）和关键基因进行分析，发现大部分通路与炎症有关。进一步研究MyD88和NFκB通路。KHES可显著减轻小鼠坐骨神经慢性收缩损伤的机械和热异常痛。KHES还抑制Myd88和p-NFκB表达的增加。NFκB通路激活剂可部分逆转KHES的抗痛觉作用，而NFκB通路抑制剂的镇痛作用与KHES相似。因此，KHES可能是治疗神经性疼痛的一种新的干预手段。
Neuropathic pain has become a global public problem and health burden. Pharmacological interventions are the primary treatment, but the drug cure rate is low with side effects. There is an urgent need to develop novel treatment approaches. High frequency electrical stimulation (KHES) has been widely applied in clinical analgesia. However, its mechanism is poorly understood. In this study, datasets related to neuropathic pain were obtained from the GEO database. The differentially expressed genes (DEGs) and key genes were analyzed through functional enrichment analysis, showing that most of the pathways involve the inflammation. The MyD88 and NFκB pathways were further studied. KHES significantly alleviated mechanical and thermal allodynia in chronic constriction injury of the sciatic nerve mice. KHES also inhibited the increase in Myd88 and p-NFκB expression. The administration of NFκB pathway activator partly reversed the antinociceptive effects of KHES, and NFκB pathway inhibitor achieved analgesic effects similar to those of KHES. Therefore, KHES might be a novel intervention for the treatment of neuropathic pain.