Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean.
Transcriptional profile of genes involved in the production of terpenes and glyceollins in response to biotic stresses in soybean.
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DOI：
10.1590/1678-4685-gmb-2019-0388
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发表时间：
2020
影响因子：
2.1
通讯作者：
Marcelino-Guimarães FC
中科院分区：
文献类型：
--
作者：
Parmezan TR;Brito Júnior SL;Carvalho K;Aquino M;Birkett M;Pickett J;Nunes EO;Abdelnor RV;Campo CBH;Marcelino-Guimarães FC
由植物产生的萜烯包括多种次级代谢产物，包括挥发性有机化合物（VOC）。萜烯VOC的产生可能会在受损后或受到细菌、真菌和昆虫等生物刺激以及随后触发植物防御反应而发生改变。这些挥发性有机化合物在植物中来自两个独立的途径：甲羟戊酸途径和甲基戊烯二磷酸途径，它们利用二甲基烯丙基和异戊烯基二磷酸形成萜类前体。豆薯层锈菌（Phakopsora pachyrhizi）真菌引起亚洲大豆锈病，限制大豆产量，并且如果不应用控制策略，则导致高达80%的损失。通过使用转录组数据集，我们研究了不同生物胁迫下甲羟戊酸途径基因的调控。我们研究了豆薯疫霉感染对携带不同抗性基因（Rpp）的基因型中参与萜类化合物和大豆抗毒素生物合成的基因的体内表达谱的影响，以及整个感染周期。此外，我们使用UPLC和UPGC分析，以评估大豆抗毒素和VOC的生产，分别确定与大豆病原体感染的反应相关的代谢物。大豆基因参与萜烯生产的调节受基因型的影响，这取决于Rpp基因，而大豆抗毒素诱导在所有基因型。此外，倍半萜被鉴定为与大豆上的锈病症状相关的潜在标记。
Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene was identified as a potential marker associated with rust symptoms on soybean.