A drop array culture for patterning adherent mouse embryonic stem cell-derived neurospheres.
A drop array culture for patterning adherent mouse embryonic stem cell-derived neurospheres.
复制标题
用于对贴壁小鼠胚胎干细胞衍生的神经球进行图案化的液滴阵列培养物。
DOI：
10.1002/term.2389
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发表时间：
2018
影响因子：
3.3
通讯作者：
Barald,KateF
中科院分区：
文献类型：
--
作者：
Dixon,AngelaR;Ramirez,Yadah;Haengel,Kathryn;Barald,KateF
用于修复受损或退化的神经系统的新的治疗方法已经加速了有效地产生源自各种干细胞来源的神经元群体的方法的发展。这些方法中的许多需要神经球的生成。这里描述了一种简单的技术，用于使用传统的塑料培养皿和图案化模板创建贴壁小鼠胚胎干细胞（mESC）衍生的神经球阵列。mESC衍生的神经球被限制在阵列内的圆形（4 mm直径）凝胶涂层区域。从mESC来源制备粘附的神经球阵列需要3天;它们可以维持在15 μl的培养基滴中，并且在培养8天后表现出广泛的神经突细化。此外，用各种分化诱导试剂证明了在阵列的选定液滴中处理粘附的神经球的潜力，随后单独分析这些神经球的基因表达、蛋白质水平和形态发育。版权所有© 2016约翰威利父子有限公司.
New therapeutic approaches for repairing an injured or degenerating nervous system have accelerated the development of methods to generate populations of neurons derived from various stem cell sources efficiently. Many of these methods require the generation of neurospheres. Here a simple technique is described for creating an array of adherent mouse embryonic stem cell (mESC)‐derived neurospheres using a conventional plastic culture dish and a patterning template. mESC‐derived neurospheres are confined to circular (4‐mm diameter), gel‐coated regions within an array. The adherent neurosphere arrays require 3 days to prepare from an mESC source; they can be maintained in 15 μl drops of medium, and exhibit extensive neurite elaboration after 8 days of cultivation. Additionally, the potential of treating the adherent neurospheres in selected drops of an array is demonstrated with a variety of differentiation‐inducing reagents and subsequently individually analysing such neurospheres for gene expression, protein levels and morphological development. Copyright © 2016 John Wiley & Sons, Ltd.