CORRELATION PROTON MAGNETIC-RESONANCE STUDIES AT 250-MHZ OF BOVINE PANCREATIC RIBONUCLEASE .1. REINVESTIGATION OF HISTIDINE PEAK ASSIGNMENTS
CORRELATION PROTON MAGNETIC-RESONANCE STUDIES AT 250-MHZ OF BOVINE PANCREATIC RIBONUCLEASE .1. REINVESTIGATION OF HISTIDINE PEAK ASSIGNMENTS
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DOI：
10.1021/bi00687a006
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发表时间：
1975-01-01
期刊：
影响因子：
2.9
通讯作者：
MARKLEY, JL
中科院分区：
文献类型：
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作者：
MARKLEY, JL
本文用质子磁共振谱（NMR）研究了在pH值为6.5和8.0时，牛胰核糖核酸酶A的四个组氨酸残基的C（2）质子的氘交换动力学。组氨酸峰的交换顺序与氚交换速率到单个组氨酸残基的比较[Ohe，M.，Matsuo，H.，Sakiyama，F.，和Narita，K.（1974），J. Biochem.（Tokyo）75，1197]支持组氨酸NMR峰H（1）和H（4）先前归属为组氨酸-105和组氨酸-48，但需要将峰H（2）和H（3）重新归属为组氨酸-119和组氨酸-12，重新命名。具有差异氘代组氨酸的核糖核酸酶A样品已用于验证组氨酸质子磁共振中交叉点的存在。
The deuterium exchange kinetics of the C (2) protons of the four histidine residues of native bovine pan-creatic ribonuclease A have been followed at pH* 6.5 and 8.0 by proton magnetic resonance spectroscopy (NMR). Comparison of the order of exchange of the histidine peaks with tritium exchange rates into individual histidine residues [Ohe, M., Matsuo, H., Sakiyama, F., and Narita, K.(1974), J. Biochem.{Tokyo) 75, 1197] supports the previ-ous assignment of histidine NMR peaks H (l) and H (4) to histidine-105 and histidine-48 but requires reassignment of peaks H (2) and H (3) to histidine-119 and histidine-12, re-spectively. Ribonuclease A samples having differentially deuterated histidines have been used to verify the existence of crossover points in the histidine proton magnetic reso-