Genetics of early Dictyostelium discoideum development.
Genetics of early Dictyostelium discoideum development.
复制标题
早期盘基网柄菌发育的遗传学。
DOI：
10.1128/mr.52.1.29-49.1988
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Kessin,RH
中科院分区：
文献类型：
--
作者：
Kessin,RH
网骨藻以细菌为食，只要有猎物，它们就会进行二分裂。一旦它们的食物耗尽或被移除，细胞开始发育并合成新的大分子，使它们能够向环腺苷酸（cAMP）的来源移动。所有使cAMP代谢和浓度梯度上升所需的器官，包括cAMP受体（106，130，131）、细胞外环核苷酸磷酸二酯酶（97，126，185）、腺苷酸环化酶（60，127，205）和cAMP分泌器官，都是从头产生的（144）。在他们的迁移到聚集中心，细胞成为粘合剂，一旦组装，他们释放出大量的新的成绩单，其产品是负责进一步分化成孢子和柄细胞（8，21，38，138）。有充分的证据表明，对cAMP的趋化性，首先在聚集过程中使用，在发育的后期，当复杂的运动导致形成迁移的蛞蝓，然后形成子实体时，会被保留以指导细胞（120，220）。Dictyosteoblasts细胞已经发明了通过将细胞表面cAMP受体连接到它们的感觉和运动装置以及它们用于基因调节的第二信使级联来协调它们的基因表达和形态发生的策略。因此，细胞外cAMP对趋化性和运动性有直接影响，对基因调控有更延迟但至关重要的影响。
Dictyostelium amoebae feed on bacteria and divide by binary fission as long as their prey is available. Once their food is exhausted or removed, the cells begin development and synthesize new macromolecules that give them the ability to move toward sourcesof cyclic adenosine 3', 5'-phosphate (cAMP). All of the apparatus required for recog-nizing cAMP and moving up a concentration gradient, including the cAMPreceptor (106, 130, 131), extracellular cyclic nucleotide phosphodiesterase (97, 126, 185), adenyl-ate cyclase (60, 127, 205), andcAMP secretory apparatus, are produced de novo (144). During their migration into centers of aggregation, the cells become adhesive, and once assembled, they unleash a large number of new transcripts, products of which are responsible for further differentiation into spore and stalk cells (8, 21, 38, 138). There is good evidence that chemotaxis toward cAMP, first used during aggregation, is retained to direct the cells during the later stages of development when complex movements lead to the formation ofa migrating slug and then to a fruiting body (120, 220). Dictyostelium cells have invented the strategem of coordinating the expression of their genes and morphogenesis by connecting a cell surface cAMP receptor to their sensory and motility apparatus and to the second messenger cascades which they use for gene regulation. Extracellular cAMP, therefore, has immediate effects on chemotaxis and motility and more delayed but crucial effects on gene regulation.