Effect of modified Widman flap surgery and systemic tetracycline on the subgingival microbiota of periodontal lesions.
Effect of modified Widman flap surgery and systemic tetracycline on the subgingival microbiota of periodontal lesions.
复制标题
改良维德曼皮瓣手术和全身四环素对牙周病变龈下微生物群的影响。
DOI：
10.1111/j.1600-051x.1988.tb01579.x
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发表时间：
1988
影响因子：
6.7
通讯作者：
Socransky,SS
中科院分区：
文献类型：
--
作者：
Haffajee,AD;Dzink,JL;Socransky,SS
33例有活动性破坏性牙周病证据的受试者接受改良Widman皮瓣手术和全身性四环素治疗（1 g/天，持续21天）。在治疗前和治疗后6个月，从其中12名受试者的41个部位采集龈下菌斑样本，用于主要可培养微生物群研究。治疗前41个采样部位的平均囊袋深度和附着水平分别为7.1 ± 2.9 mm和7.7 ± 3.2 mm，治疗后为4.8 ± 2.3 mm和6.2 ± 3.4 mm。黑素生成性和V.在治疗后采集的样本中，更频繁地检测到细小病毒，而S.中间S. morbillorum、S. uberisandW.治疗后直肠癌检出率较低。治疗前7个部位检出放线菌阳性，治疗后1个部位检出放线菌阳性。B.牙龈炎和B.中间体几乎没有变化。平均水平的Aetinomylop.，A. actinomycetemcomitans、伴放线菌B. gingivalis，B. B.中间S.麻疹uberisandW.治疗后直肠B超平均水平下降，melaninogenicus、S. mitis，S. sanguisII和V.其中以小弧菌的增加最大，占菌群的8.2%。在第二阶段的研究中，龈下菌斑样本从94个网站在33个治疗的主题进行了分析，主要培养技术。作为治疗的结果，24个部位表现出附着损失>2 mm，23个部位表现出“增益”>2 mm，其余47个部位被认为是不变的。将处理部位的微生物结果与100个“活性”部位的主要可培养微生物进行比较（即，在治疗前失去> 2.5mm附着的部位）。S. mitis，S. sanguisI和II和V。治疗后附着没有变化或“增加”的小蛋白位点。actinomycetemcomitans、伴放线菌B. Alpenthus，B. gingivalis，B. B.中间肌B。melaninogenicus、P. microsandIV.直肠癌在这些部位常未检出。actinomycetemcomitans、伴放线菌B. Alpenthus，B. gingivalis，B. B. intermedius，B. melaninogenicus、P. micros、S. intermediusandW.治疗后活动部位和/或失去附着部位的直肠平均恢复水平最高。放线菌属则相反，S. mitis，S. sanguisI和II和V。小的
33 subjects with evidence of active destructive periodontal disease were treated by modified Widman flap surgery and systemic tetracycline (1 g/day for 21 days). Subgingival plaque samples were taken from 41 sites in 12 of these subjects before and 6 months after therapy for predominant cultivable microbiota studies. Mean pocket depth and attachment levels in the 41 sampled sites were 7.1 ± 2.9 mm and 7.7 ± 3.2 mm prior to therapy and 4.8 ± 2.3 mm and 6.2 ± 3.4 mm after therapy.B. melaninogenicusandV. parvulawere more frequentlydetectedin samples taken after therapy, whileS. intermedius, S. morbillorum, S. uberisandW. rectawere less frequentlydetectedafter therapy.A. actinomycetemcomitanswasdetectedin 7 sites pretherapy and 1 site post therapy. The frequency ofdetectionofB. gingivalisandB. intermediuswas virtually unchanged. Themean levelsof theAetinomycessp.,A. actinomycetemcomitans, B. gingivalis, B. intermedius, S. morbillorum.S. uberisandW. rectawere decreased after therapy, while themean levelsofB. melaninogenicus, S. mitis, S. sanguisII andV. parvulawere increased after therapy.V. parvulashowed the greatest increase to 8.2% of the microbiota. In the second phase of the study, subgingival plaque samples from 94 sites in the 33 treated subjects were analyzed by predominant cultivable techniques. As a result of therapy, 24 sites exhibited attachment loss >2 mm, 23 sites exhibited “gain” >2 mm and the remaining 47 sites were considered to be unchanging. The microbiological findings from the treated sites were compared with the predominant cultivable microbiotas of 100 “active” sites (i.e., sites which lost >2.5 mm of attachment prior to therapy). There was an increase in the frequency ofdetectionofS. mitis, S. sanguisI and II andV. parvulain sites which showed no change or “gain” in attachment after therapy.A. actinomycetemcomitans, B. forsythus, B. gingivalis, B. intermedius B. melaninogenicus, P. microsandIV. rectaweredetectedless frequently in these sites.A. actinomycetemcomitans, B. forsythus, B. gingivalis, B. intermedius, B. melaninogenicus, P. micros, S. intermediusandW. rectawere recovered in highestmeanlevels in active sites and or sites which lost attachment after therapy. The opposite pattern was observed for theActinomycessp.,S. mitis, S. sanguisI and II andV. parvula.