DHICA OXIDASE ACTIVITY OF TRP1 AND INTERACTIONS WITH OTHER MELANOGENIC ENZYMES
DHICA OXIDASE ACTIVITY OF TRP1 AND INTERACTIONS WITH OTHER MELANOGENIC ENZYMES
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DOI：
10.1111/j.1600-0749.1994.tb00054.x
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发表时间：
1994-08-01
期刊：
影响因子：
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通讯作者：
HEARING, VJ
中科院分区：
文献类型：
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作者：
KOBAYASHI, T;URABE, K;HEARING, VJ
酪氨酸酶相关蛋白1 （TRP1）在小鼠中定位于棕色位点。尽管TRP1的具体功能一直存在争议，但其结构基因的突变导致棕色黑色素而不是黑色黑色素的形成。我们通过免疫亲和纯化TRP1蛋白和将其基因转染成纤维细胞来研究其特性，从而研究了TRP1的黑色素生成功能。我们发现TRP1具有氧化DHICA的能力，DHICA是一种来自DOPAchrome的黑色素生成中间体。此外，TRP1具有与酪氨酸酶相互作用的能力，显著稳定酪氨酸酶的催化功能。
Tyrosinase-related protein 1 (TRP1) maps to the brown locus in mice. Although the specific function of TRP1 has been in dispute, mutations in its structural gene result in the formation of brown rather than black melanin. We have investigated the melanogenic function of TRP1 by using immune-affinity purification of the protein and also by using transfection of its gene into fibroblasts to study its characteristics. We show that TRP1 has the ability to oxidize DHICA, a melanogenic intermediate derived from DOPAchrome. In addition, TRP1 has the ability to interact with tyrosinase and significantly stabilize the latter's catalytic function.