Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
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DOI：
10.2147/ijn.s191878
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
Zarrabi, Ali
中科院分区：
文献类型：
--
作者：
Katebi, Samira;Esmaeili, Abolghasem;Zarrabi, Ali
背景：研究促进神经退行性疾病后神经再生恢复的药物一直是神经科学领域的重要课题。神经生长因子（NGF）和槲皮素作为潜在的类黄酮在帕金森、阿尔茨海默等退行性疾病的治疗中具有潜在的应用价值。材料和方法：MTT法分别在24h、48h和72h检测超顺磁性氧化铁纳米颗粒（SPIONs）和槲皮素的细胞毒性。我们将NGF和槲皮素与不同浓度的SPIONs作为新化合物联合使用，研究它们对PC12细胞神经元分支形态发生的影响。结果：形态学分析显示PC12细胞在槲皮素溶液中培养后，神经突长度显著增加（P < 0.001）。我们发现，将PC12细胞暴露于槲皮素和SPIONs后，显著促进了神经突的生长，并增加了神经元分支树的复杂性。此外，槲皮素和SPIONs处理后，这些细胞中β 3-微管蛋白的表达水平更高。结论：不同的摄影分析表明，氧化铁纳米颗粒是提高NGF效率的一个重要因素，通过提高细胞活力、细胞附着和神经突生长，槲皮素是这些现象的促进剂。使用槲皮素- spion复合物作为提高NGF功效和活性的合适方法，为大量的神经元修复治疗打开了一扇新的窗口。
Background: The investigation of agents promoting recovery of nerve regeneration following neurodegenerative diseases has been the most important issue in neuroscience. Nerve growth factor (NGF) and quercetin as potential flavonoids could possibly have therapeutic applications in the field of degenerative diseases such as Parkinson and Alzheimer.Materials and methods: The MTT assay was done at 24, 48, and 72 hours to examine the cytotoxicity of superparamagnetic iron oxide nanoparticles (SPIONs) and quercetin. We combined NGF and quercetin with different concentrations of SPIONs as novel compounds to study their effect on neuronal branching morphogenesis of PC12 cells.Results: Morphological analysis showed a significant growth (P < 0.001) in neurite length when PC12 cells were incubated in quercetin solution. We found a significant neurite outgrowth promotion and an increase in the complexity of the neuronal branching trees after exposing PC12 cells to both quercetin and SPIONs. In addition, a higher level of beta 3-tubulin expression was observed in these cells when treated with both quercetin and SPIONs.Conclusion: Different photographic analyses indicated that iron oxide nanoparticles function as an important factor in order to improve the efficiency of NGF through improving cell viability, cell attachment, and neurite outgrowth in the shelter of quercetin as an accelerator of these phenomena. The use of the quercetin-SPION complex as a suitable method for improving NGF efficacy and activity opens a novel window for substantial neuronal repair therapeutics.