Analytical and biological methods for probing the blood-brain barrier.
Analytical and biological methods for probing the blood-brain barrier.
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DOI：
10.1146/annurev-anchem-062011-143002
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Lunte SM
中科院分区：
文献类型：
--
作者：
Kuhnline Sloan CD;Nandi P;Linz TH;Aldrich JV;Audus KL;Lunte SM
血脑屏障(BBB)是外周和中枢神经系统之间的重要接口。它保护大脑免受有害物质的渗透，并调节有益的内源性物质从血液渗透到脑细胞外液中。它还可能成为开发针对中枢神经系统的药物的主要障碍。已经开发了几种方法来研究药物、多肽和内源性化合物在血脑屏障的运输和代谢。体内方法包括静脉注射、脑灌注、正电子发射断层扫描和微透析取样。研究人员还开发了体外细胞培养模型，可以用来研究血脑屏障的运输和新陈代谢，而不会出现全身参与的并发症。所有这些方法都需要灵敏和选择性的分析方法来监测BBB中感兴趣的化合物的运输和代谢。
The blood-brain barrier (BBB) is an important interface between the peripheral and central nervous systems. It protects the brain against the infiltration of harmful substances and regulates the permeation of beneficial endogenous substances from the blood into the extracellular fluid of the brain. It can also present a major obstacle in the development of drugs that are targeted for the central nervous system. Several methods have been developed to investigate the transport and metabolism of drugs, peptides, and endogenous compounds at the BBB. In vivo methods include intravenous injection, brain perfusion, positron emission tomography, and microdialysis sampling. Researchers have also developed in vitro cell-culture models that can be employed to investigate transport and metabolism at the BBB without the complication of systemic involvement. All these methods require sensitive and selective analytical methods to monitor the transport and metabolism of the compounds of interest at the BBB.
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DOI：
10.1016/j.jpba.2006.10.005
发表时间：
2007-03-12
影响因子：
3.4
作者：
Chappa, Arvind K.;Cooper, Joshua D.;Lunte, Susan M.
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/s1569-7339(06)16029-4
发表时间：
2007-01-01
期刊：
HANDBOOK OF MICRODIALYSIS: METHODS, APPLICATIONS AND PERSPECTIVES
影响因子：
--
作者：
de Lange, Elizabeth C. M.
通讯作者：
de Lange, Elizabeth C. M.
影响因子：
3.9
作者：
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通讯作者：
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影响因子：
3.8
作者：
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通讯作者：
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