MONONUCLEAR-CELL INFILTRATION IN OVARIAN-CANCER .1. INFLAMMATORY - CELL INFILTRATES FROM TUMOR AND ASCITES MATERIAL
MONONUCLEAR-CELL INFILTRATION IN OVARIAN-CANCER .1. INFLAMMATORY - CELL INFILTRATES FROM TUMOR AND ASCITES MATERIAL
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DOI：
10.1038/bjc.1982.114
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发表时间：
1982-01-01
影响因子：
8.8
通讯作者：
WALTON, L
中科院分区：
文献类型：
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作者：
HASKILL, S;BECKER, S;WALTON, L
手术中获得的恶性积液和肿瘤组织为研究各种炎性细胞在人类卵巢癌预后中的预后价值提供了材料。腹水主要含有炎性细胞;单个和成簇的肿瘤细胞是次要成分。肿瘤细胞通常在分散的固体物质中过量。一些患者在其实体瘤中具有显著比例的淋巴细胞和巨噬细胞，并且这些患者总是对治疗有反应。单位重力下的沉降速度分离提供了2个炎症细胞群。一种由大小与患者血液中的单核细胞相似的单核细胞组成：另一种由1个或多个大的巨噬细胞群组成，其形态和酶标记物与血液单核细胞和彼此不同。与患者血液（60%）相比，T淋巴细胞在腹水部分（78%）和肿瘤来源的单核细胞部分（71%）中富集。T细胞亚群的特征是ANAE [酸性萘乙酸酯酶]反应性显着耗尽肿瘤浸润部分（17%）相对于腹水（62%）或患者血液（51%）。酯酶阳性单核细胞样细胞在肿瘤浸润部分（17%）比腹水（7%）或血液（12%）更常见。B淋巴细胞在实体瘤中很少见，在腹水中很难评估。组织细胞样巨噬细胞存在于包含固体和腹水材料的较高速度的肿瘤细胞部分中。不同患者之间以及同一个体的肿瘤和腹水之间浸润细胞的差异显著。
Malignant effusions and tumor tissue obtained at surgery provided material for a study of the prognostic value of the various inflammatory cells in the prognosis of human ovarian cancer. Ascitic fluids predominantly contained inflammatory cells; tumor cells, both singly and in clusters, were a minor component. Tumor cells were usually in excess in dispersed solid material. Some patients had significant proportions of lymphocytes and macrophages in their solid tumor, and these patients invariably responded to therapy. Sedimentation-velocity separation at unit gravity provided 2 populations of inflammatory cells. One consisted of mononuclear cells similar in size to those in the patients blood: the other consisted of 1 or more large macrophage populations, distinct in morphology and enzymatic markers from both blood monocytes and each other. T lymphocytes were enriched in ascites fractions (78%) and in the tumor-derived mononuclear fraction (71%) compared to patient blood (60%). The T-cell subset characterized by ANAE [acid naphthyl-acetate esterase] reactivity was markedly depleted in the tumor-infiltrating fraction (17%) relative to either the ascites (62%) or patient blood (51%). Esterase-positive monocyte-like cells were more frequent in the tumor-infiltrating fraction (17%) than in ascites (7%) or blood (12%). B lymphocytes were infrequent in solid tumors and difficult to assess in ascites. Histiocyte-like macrophages were present in the higher-velocity tumor-cell containing fractions of both solid and ascitic material. The variation in infiltrating cells between patients and between tumor and ascites of the same individual was marked.