Transport of sugars and amino acids in bacteria. 3. Studies on the restoration of active transport.
Transport of sugars and amino acids in bacteria. 3. Studies on the restoration of active transport.
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细菌中糖和氨基酸的运输。
DOI：
10.1016/s0021-9258(18)93387-9
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
Y. Anraku
中科院分区：
文献类型：
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作者：
Y. Anraku
关于休克细胞对主动转运抑制剂的敏感性，已经进行了检测。它们的行为与未受电击的细胞相似，只是它们的运输能力降低了。对主动运输的恢复过程进行了研究。代谢能量似乎是恢复休克细胞摄取能力所必需的。有证据表明，半乳糖和亮氨酸结合蛋白可以与休克细胞重新结合。这一过程表现出饱和动力学，并导致输送能力的增加。我们推测，存在能够与结合蛋白相互作用的特定部位。除了结合蛋白外，我们还发现休克液中另一个不可透析的因子在恢复运输方面具有活性。该因子由低浓度的硫酸铵沉淀而成，不具有结合性质。它的活性很高，只有当该因子与结合蛋白结合使用时，才能恢复到接近原始水平的转运。
Shocked cells have been examined with respect to their sensitivity to inhibitors of active transport. They behave similarly to unshocked cells except that their transport ability is reduced. The process of restoration of active transport has been studied. Metabolic energy appears to be necessary to restore the uptake ability of shocked cells.Evidence is presented which suggests that the galactose- and leucine-binding proteins can become reassociated with the shocked cells. The process shows saturation kinetics and results in an increase in transport ability. We speculate that there are specific sites capable of interacting with the binding proteins.In addition to binding protein, another nondialyzable factor in shock fluid is found to be active in restoration of transport. This factor is precipitated by low concentrations of ammonium sulfate and does not have binding properties. Its activity is substantial and restoration of transport to nearly the original level can be shown only when this factor is used in combination with binding protein.