The neurochemical characterisation of hypothalamic pathways projecting polysynaptically to brown adipose tissue in the rat
The neurochemical characterisation of hypothalamic pathways projecting polysynaptically to brown adipose tissue in the rat
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DOI：
10.1016/s0306-4522(01)00555-3
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
McKinley, MJ
中科院分区：
文献类型：
--
作者：
Oldfield, BJ;Giles, ME;McKinley, MJ
瘦素和一系列新的厌食和食欲肽的鉴定已经将注意力集中在参与食物摄入发生和产热反射控制的神经回路上。在这里，嗜神经病毒伪狂犬病已被用于结合免疫细胞化学定位的各种神经活性肽和受体，以更好地定义在大鼠下丘脑的途径，多突触的主要产热终点，棕色脂肪组织。最初在星状神经节中检测到感染的神经元，然后在脊髓中，随后在脑干和下丘脑中出现三级运动前神经元。在下丘脑内，这些存在于室旁核、外侧下丘脑、穹窿周围区和视交叉后核。在稍长的生存时间病毒感染的神经元出现在弓状核和背内侧下丘脑。神经质在视交叉后核和邻近的外侧弓状核中投射到棕色脂肪组织，表达可卡因和安非他明调节的转录物、前阿黑皮素和瘦素受体。下丘脑外侧（传统上与促进进食有关的部位）的神经质投射到棕色脂肪组织，其中大量含有黑色素浓缩激素和食欲素A和B。这些数据提供了有助于能量消耗调节的解剖学框架的一部分。(C)2002年由Elsevier Science Ltd.代表IBRO出版。
The identification of leptin and a range of novel anorectic and orexigenic peptides has focussed attention on the neural circuitry involved in the genesis of food intake and the reflex control of thermogenesis. Here, the neurotropic virus pseudorabies has been utilised in conjunction with the immunocytochemical localisation of a variety of neuroactive peptides and receptors to better define the pathways in the rat hypothalamus directed polysynaptically to the major thermogenic endpoint, brown adipose tissue. Infected neurones were detected initially in the stellate ganglion, then in the spinal cord followed by the appearance of third-order premotor neurones in the brainstem and hypothalamus. Within the hypothalamus these were present in the paraventricular nucleus, lateral hypothalamus, perifornical region, and retrochiasmatic nucleus. At slightly longer survival times virus-infected neurones appeared in the arcuate nucleus and dorsomedial hypothalamus. Neurotics in the retrochiasmatic nucleus and in the adjacent lateral arcuate nucleus which project to the brown adipose tissue express cocaine- and amphetamine-regulated transcript, pro-opiomelanocortin and leptin receptors. Neurotics in the lateral hypothalamus, a site traditionally associated with the promotion of feeding, project to brown adipose tissue and large numbers of these contained melanin-concentrating hormone and orexin A and B. These data provide part of an anatomical framework which subserves the regulation of energy expenditure. (C) 2002 Published by Elsevier Science Ltd. on behalf of IBRO.