Saccharomyces cerevisiae vacuole in zinc storage and intracellular zinc distribution.
Saccharomyces cerevisiae vacuole in zinc storage and intracellular zinc distribution.
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酿酒酵母液泡中锌的储存和细胞内锌的分布。
DOI：
10.1128/ec.00077-07
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Eide,DavidJ
中科院分区：
文献类型：
--
作者：
Simm,Claudia;Lahner,Brett;Salt,David;LeFurgey,Ann;Ingram,Peter;Yandell,Brian;Eide,DavidJ
Previous studies of the yeastSaccharomyces cerevisiaeindicated that the vacuole is a major site of zinc storage in the cell. However, these studies did not address the absolute level of zinc that was stored in the vacuole nor did they examine the abundances of stored zinc in other compartments of the cell. In this report, we describe an analysis of the cellular distribution of zinc by use of both an organellar fractionation method and an electron probe X-ray microanalysis. With these methods, we determined that zinc levels in the vacuole vary with zinc status and can rise to almost 100 mM zinc (i.e., 7 × 108atoms of vacuolar zinc per cell). Moreover, this zinc can be mobilized effectively to supply the needs of as many as eight generations of progeny cells under zinc starvation conditions. While the Zrc1 and Cot1 zinc transporters are essential for zinc uptake into the vacuole under steady-state growth conditions, additional transporters help mediate zinc uptake into the vacuole during “zinc shock,” when zinc-limited cells are resupplied with zinc. In addition, we found that other compartments of the cell do not provide significant stores of zinc. In particular, zinc accumulation in mitochondria is low and is homeostatically regulated independently of vacuolar zinc storage. Finally, we observed a strong correlation between zinc status and the levels of magnesium and phosphorus accumulated in cells. Our results implicate zinc as a major determinant of the ability of the cell to store these other important nutrients.
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