The Arabidopsis transcription factor NAI1 is required for enhancing the active histone mark but not for removing the repressive mark on PYK10, a seedling–specific gene upon embryonic-to-postgerminative developmental phase transition.
The Arabidopsis transcription factor NAI1 is required for enhancing the active histone mark but not for removing the repressive mark on PYK10, a seedling–specific gene upon embryonic-to-postgerminative developmental phase transition.
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拟南芥转录因子 NAI1 是增强活性组蛋白标记所必需的，但不是消除幼苗 PYK10 上的抑制标记所必需的
DOI：
10.1080/15592324.2015.1105418
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
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作者：
Yoshii;M.;Yamamoto;A.;Kagaya;Y.;Takeda S. and Hattori;T.
我们最近发现，在种子成熟调节突变体（如lec1）中，种子特异性基因PYK10在从胚胎到萌发后阶段和胚胎发生过程中以细胞为单位的方式表达，并在这一过程中涉及表观遗传机制。本文研究了pyk10表达所需的NAI1转录因子在pyk10发育开关中的作用。pyk10 - egfpinlec1胚胎在ai1背景下仍能观察到细胞间的发生，但水平大大降低。抑制组蛋白标记H3K27三甲基化水平的降低通常发生在胚胎发育后阶段1。然而，伴随活性标记水平的增加，野生型中观察到的H3K4三甲基化在nai1中显著受损。这些结果表明，pyk10在发育相变中的开关涉及染色质状态变化的两个可分离步骤。
We have recently shown that the expression onset of a seedling-specific gene,PYK10, occurs in a cell-by-cell manner upon the transition from the embryonic to the postgerminative phase and during embryogenesis in seed maturation regulator mutants such aslec1, and implicated epigenetic mechanisms in the process. Here, the role of the NAI1 transcription factor required forPYK10expression in the developmental switching ofPYK10was investigated. The cell-by-cell onset ofPYK10-EGFPinlec1embryo was still observed in thenai1background, but at greatly reduced levels. Decreases in the level of the repressive histone mark, H3K27 trimethylation observed upon the transition to the postgeminative phase normally occurred innai1. However, concomitant increases in the level of the active mark, H3K4 trimethylation observed in wild type was significantly compromised innai1. These results indicate that the switching ofPYK10upon developmental phase transition involves 2 separable steps of chromatin state change.