Interactome for auxiliary splicing factor U2AF(65) suggests diverse roles.
Interactome for auxiliary splicing factor U2AF(65) suggests diverse roles.
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辅助剪接因子U2AF（65）的互动组提出了各种作用。
DOI：
10.1016/j.bbagrm.2009.06.002
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发表时间：
2009-06
影响因子：
4.7
通讯作者：
Schmidt, Edward E.
中科院分区：
文献类型：
--
作者：
Prigge, Justin R.;Iverson, Sonya V.;Siders, Ashley M.;Schmidt, Edward E.
U2小核核糖核蛋白辅助因子（U2AF）是剪接机制的重要组成部分，由35kD的U2AF 35（U2AF 1）和65kD的U2AF 65（U2AF 2）两个蛋白亚基组成。U2AF与该机制内的各种剪接因子相互作用。在这里，我们扩展了已知与U2AF 65相互作用的哺乳动物剪接因子的列表，以及未知参与与U2AF 65相互作用的剪接的核蛋白的列表。利用酵母双杂交系统，我们发现了14个U2AF65相互作用蛋白。通过鉴定五种已知的U2AF 65相互作用蛋白，包括其异二聚体伴侣U2AF 35，证实了筛选的有效性。除了结合这些已知的伙伴，我们发现了以前未被识别的U2AF 65与四个剪接相关蛋白（DDX 39，SFRS 3，SFRS 18，SNRPA），两个锌指蛋白（ZFP 809和ZC3H11A），一个U2AF 65同源物（RBM39）和两个其他调节蛋白（DAXX和SERBP1）的相互作用。我们报告了这些蛋白质与U2AF65的哪些区域相互作用，并讨论了它们在调节前体mRNA剪接、3 '端mRNA加工和U2AF65亚核定位中的潜在作用。这些发现表明U2AF 65在剪接和非剪接功能中的作用扩大。
U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) is an essential component of the splicing machinery that is composed of two protein subunits, the 35 kD U2AF35 (U2AF1) and the 65 kD U2AF65 (U2AF2). U2AF interacts with various splicing factors within this machinery. Here we expand the list of mammalian splicing factors that are known to interact with U2AF65 as well as the list of nuclear proteins not known to participate in splicing that interact with U2AF65. Using a yeast two-hybrid system, we found fourteen U2AF65-interacting proteins. The validity of the screen was confirmed by identification of five known U2AF65-interacting proteins, including its heterodimeric partner, U2AF35. In addition to binding these known partners, we found previously unrecognized U2AF65 interactions with four splicing related proteins (DDX39, SFRS3, SFRS18, SNRPA), two zinc finger proteins (ZFP809 and ZC3H11A), a U2AF65 homolog (RBM39), and two other regulatory proteins (DAXX and SERBP1). We report which regions of U2AF65 each of these proteins interacts with and we and discuss their potential roles in regulation of pre-mRNA splicing, 3’-end mRNA processing, and U2AF65 sub-nuclear localization. These findings suggest expanded roles for U2AF65 in both splicing and non-splicing functions.
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影响因子：
3.3
作者：
Izquierdo, Jose M.
通讯作者：
Izquierdo, Jose M.
影响因子：
12.3
作者：
Gama-Carvalho, Margarida;Barbosa-Morais, Nuno L.;Carmo-Fonseca, Maria
通讯作者：
Carmo-Fonseca, Maria
影响因子：
14.9
作者：
Chatr-aryamontri, Andrew;Ceol, Arnaud;Palazzi, Luisa Montecchi;Nardelli, Giuliano;Schneider, Maria Victoria;Castagnoli, Luisa;Cesareni, Gianni
通讯作者：
Cesareni, Gianni
影响因子：
16.8
作者：
Corsini, Lorenzo;Bonnal, Sophie;Sattler, Michael
通讯作者：
Sattler, Michael
影响因子：
64.5
作者：
Kielkopf, CL;Rodionova, NA;Burley, SK
通讯作者：
Burley, SK