The effect of oxygen tension on the in vitro assay of human osteoblastic connective tissue progenitor cells.
The effect of oxygen tension on the in vitro assay of human osteoblastic connective tissue progenitor cells.
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氧张力对人成骨结缔组织祖细胞体外测定的影响。
DOI：
10.1002/jor.20666
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Muschler,GeorgeF
中科院分区：
文献类型：
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作者：
Villarruel,SandraM;Boehm,CynthiaA;Pennington,Mark;Bryan,JasonA;Powell,KimerlyA;Muschler,GeorgeF
结缔组织祖细胞（CTPs）被定义为存在于天然组织中并且能够增殖和分化成一种或多种结缔组织表型的干细胞和祖细胞的异质集合。CTPs在组织形成、修复和重塑中发挥重要作用。因此，CTP患病率和生物学潜力的体外测定具有重要的科学和临床意义。本研究评价了氧张力作为优化人CTP定量测定体外条件的重要变量。从20名人类受试者中采集骨髓穿刺液，并在1、5、10和20%的环境氧分压下使用已建立的培养基条件进行培养。评估集落形成效率（CFE）、增殖和集落密度。CFE和增殖在5%O2时最大。传统的条件下，使用大气中的氧分压（20%O2）减少CFE多达32%。CFE和增殖在1%O2低于5%O2，但在20%O2的情况下，这表明CTPs是相对弹性的缺氧条件下，一个事实，可能是相关的伤口修复功能和他们的组织工程应用中的潜在用途，涉及移植到中度至重度缺氧的设置。这些数据表明CTP定量测定的优化将需要控制氧张力。© 2008骨科研究学会。由威利期刊公司出版J Orthop Res 26：1390-1397，2008
Connective tissue progenitors (CTPs) are defined as the heterogeneous set of stem and progenitor cells that reside in native tissues and are capable of proliferation and differentiation into one or more connective tissue phenotypes. CTPs play important roles in tissue formation, repair, and remodeling. Therefore, in vitro assays of CTP prevalence and biological potential have important scientific and clinical relevance. This study evaluated oxygen tension as an important variable in optimizing in vitro conditions for quantitative assays of human CTPs. Bone marrow aspirates were collected from 20 human subjects and cultured using established medium conditions at ambient oxygen tensions of 1, 5, 10, and 20%. Colony‐forming efficiency (CFE), proliferation, and colony density were assessed. CFE and proliferation were greatest at 5% O2. Traditional conditions using atmospheric oxygen tension (20% O2) reduced CFE by as much as 32%. CFE and proliferation at 1% O2were less than 5% O2but comparable to that seen at 20% O2, suggesting that CTPs are relatively resilient under hypoxic conditions, a fact that may be relevant to their function in wound repair and their potential use in tissue engineering applications involving transplantation into settings of moderate to severe hypoxia. These data demonstrate that optimization of quantitative assays for CTPs will require control of oxygen tension. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:1390–1397, 2008