Non-invasive quantification of resin-dentin interfacial gaps using optical coherence tomography: Validation against confocal microscopy
Non-invasive quantification of resin-dentin interfacial gaps using optical coherence tomography: Validation against confocal microscopy
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DOI：
10.1016/j.dental.2011.05.003
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发表时间：
2011-09-01
期刊：
影响因子：
5
通讯作者：
Sumi, Yasunori
中科院分区：
文献类型：
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作者：
Bakhsh, Turki A.;Sadr, Alireza;Sumi, Yasunori
目标。无论何种原因，在牙齿-修复体界面处形成间隙都可能导致治疗失败；对这些缺陷进行无损评估和监测是很重要的。这项体外研究的目的是使用非侵入性技术评估牙齿修复界面；扫描源光学相干层析成像（SS-OCT）和共聚焦激光扫描显微镜（CLSM）证实。在人前磨牙咬合面制备直径为3mm、深度为1.5 mm的圆柱形i类牙槽。每个腔体都使用一体化粘合系统（Clearfil Tri-S Bond）和三种复合材料中的一种进行修复；Majesty Posterior， AP-X和Majesty LV（均由日本Kuraray Medical公司生产）。在1319 nm中心波长下，通过SS-OCT获得整个修复腔的10张连续横切面图像，然后在CLSM下对这些位置的标本进行修整、抛光和观察。利用图像分析软件在牙本质-树脂界面处检测到明显的信号强度峰。clsm结果也证实了界面间隙的存在和尺寸。沿界面方向SS-OCT信号强度的增加与CLSM检测到的界面间隙相对应。实际检测到的间隙尺寸在26 μ m ~ 1.9 mm之间，通用复合材料APX的界面间隙最小。SS-OCT成像技术可用于复合材料修复体底部微米间隙的无创检测和量化，有可能成为实验室研究和临床应用中复合材料修复体的监测工具。(C) 2011年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objectives. Regardless of the cause, gap formation at the tooth-restoration interface may result in treatment failure; non-destructive assessment and monitoring of these defects are important. The aim of this in vitro study is to assess the tooth-restoration interface using a non-invasive technique; swept source optical coherence tomography (SS-OCT) and to confirm the findings with confocal laser scanning microscope (CLSM).Methods. Cylindrical class-I cavities (3 mm in diameter and 1.5 mm in depth) were prepared in the occlusal surface of human premolars. Each cavity was restored using an all-in-one adhesive system (Clearfil Tri-S Bond) and one of the three types of composites placed in bulk; Majesty Posterior, AP-X and Majesty LV (all by Kuraray Medical, Japan). Ten serial cross-sectional images of the whole restored cavity were obtained by SS-OCT at 1319 nm center wave length, to which locations the specimens were later trimmed, polished and observed under CLSM. An image analysis software was used to detect significant peaks in the signal intensity at the resin-dentin interface of the cavity floor. The presence and dimensions of gaps at the interface were also confirmed by CLSM.Results. Increased SS-OCT signal intensity along the interface corresponded well to the interfacial gaps detected by CLSM. The actual gap size detected ranged from 26 mu m to 1.9 mm in length, and the universal composite APX showed lowest interfacial gaps.Conclusion. SS-OCT imaging technology can be used to non-invasively detect and quantify micrometer gaps at the bottom of composite restorations, and potentially become a monitoring tool for composite restorations both in the laboratory research, and in the clinics. (C) 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.