Effects of GABA on acutely isolated neurons from the gustatory zone of the rat nucleus of the solitary tract
Effects of GABA on acutely isolated neurons from the gustatory zone of the rat nucleus of the solitary tract
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DOI：
10.1093/chemse/23.6.683
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发表时间：
1998-12-01
期刊：
影响因子：
3.5
通讯作者：
Bradley, RM
中科院分区：
文献类型：
--
作者：
Du, JH;Bradley, RM
采用全细胞电流钳记录技术，研究了急性分离的孤束嘴侧核（rNST）神经元对GABA受体激动剂和拮抗剂的反应。分离的神经元保持其形态，并可分为多极，细长和卵圆形细胞类型。大多数rNST神经元（97%），包括所有三种细胞类型，响应GABA与膜超极化和输入电阻的减少。GABA（A）受体激动剂蝇蕈醇以浓度依赖性方式降低神经元输入阻力，而GABA（B）受体激动剂巴氯芬对任何测试的神经元都没有影响。GABA和蝇蕈醇的逆转电位均为-75 mV。GABA竞争性拮抗剂印防己毒素和GABA（A）受体拮抗剂荷包牡丹碱均以浓度依赖性方式阻断GABA的作用。这些结果表明，GABA激活所有神经元的rNST和抑制性突触活动是重要的味觉和体感信息的脑干处理。
Responses of acutely isolated neurons from the rostral nucleus of the solitary tract (rNST) to GABA receptor agonists and antagonists were investigated using whole-cell recording in current clamp mode. The isolated neurons retain their morphology and can be divided into multipolar, elongate and ovoid cell types. Most rNST neurons (97%), including all three cell types, respond to GABA with membrane hyperpolarization and a reduction in input resistance. The GABA(A) receptor agonist muscimol reduces neuronal input resistance in a concentration-dependent manner, whereas the GABA(B) receptor agonist baclofen had no effect on any of the neurons tested. The GABA and muscimol reversal potentials were both found to be -75 mV. Both the GABA competitive antagonist picrotoxin and the GABA(A) receptor antagonist bicuculline block the effect of GABA in a concentration-dependent manner. These results suggest that GABA activates all neurons in the rNST and that inhibitory synaptic activity is important in brainstem processing of gustatory and somatosensory information.