Vasorelaxing Effect of Resveratrol on Bovine Retinal Arteries.
Vasorelaxing Effect of Resveratrol on Bovine Retinal Arteries.
复制标题
白藜芦醇对牛视网膜动脉的血管舒张作用。
DOI：
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发表时间：
2016
影响因子：
4.4
通讯作者：
J. van de Voorde
中科院分区：
文献类型：
--
作者：
L. Vanden Daele;Charlotte Boydens;B. Pauwels;J. van de Voorde
目的
白藜芦醇是一种红酒多酚，可引起血管松弛，在治疗或预防血液流动受损的眼病方面可能有兴趣。本研究观察了白藜芦醇(顺式和反式)对牛视网膜动脉的松弛作用，探讨了白藜芦醇的松弛机制及其对视网膜松弛因子(RRF)松弛作用的影响。
方法
将分离的牛视网膜动脉安装到线状肌图仪中进行等长张力测量。绘制了顺式和反式白藜芦醇的浓度-反应曲线，以及白藜芦醇在血管内皮细胞或不同抑制剂存在或不存在时的浓度-反应曲线。还比较了RRF在白藜芦醇孵育和不孵育的情况下所引起的松弛。
结果
两种白藜芦醇异构体都引起了类似的强烈浓度依赖的松弛。去除内皮细胞或阻断内皮依赖通路不会改变松弛。此外，除120 mM K+Krebs林格重碳酸盐溶液外，K+通道阻滞剂并不降低舒张性。佛波醇12-肉豆蔻酸酯13-乙酸酯和佛波醇12，13-二丁酸酯部分阻断了这种松弛，对血红素加氧酶-1的抑制也是如此。阻断腺苷环化酶、AMP激活的蛋白激酶、雌激素受体、sirtuin1或肌浆网/内质网钙ATPase均不起作用。白藜芦醇温育不改变RRF引起的松弛。
结论
顺式和反式白藜芦醇对牛视网膜动脉的松弛作用相似，且呈浓度依赖性。主要的松弛机制尚不清楚，但可能涉及K+通道、一氧化碳和肌球蛋白磷酸酶途径。白藜芦醇对RRF无影响。
PURPOSE
Resveratrol is a red wine polyphenol that causes vasorelaxation, which could be of interest in the treatment or prevention of eye diseases with an impaired blood flow. In this study, the vasorelaxant capacity of resveratrol (cis and trans) on bovine retinal arteries, its vasorelaxing mechanism, and its influence on the relaxation induced by the retinal relaxing factor (RRF) were examined.
METHODS
Isolated bovine retinal arteries were mounted into wire myographs for isometric tension measurements. Concentration-response curves of cis- and trans-resveratrol and concentration-response curves of resveratrol in the absence or presence of the endothelium or different inhibitors were constructed. Relaxations elicited by the RRF with and without resveratrol incubation were also compared.
RESULTS
Both resveratrol isomers caused a similar strong concentration-dependent relaxation. Removal of the endothelium or blocking endothelium-dependent pathways did not change the relaxation. Also, K+ channel blockers did not reduce the relaxation, except the 120 mM K+ Krebs Ringer bicarbonate solution. Phorbol 12-myristate 13-acetate and phorbol 12,13-dibutyrate blocked the relaxation partially and so did the inhibition of heme oxygenase-1. Blocking adenylyl cyclase, AMP-activated protein kinase, estrogen receptors, sirtuin 1, or sarco/endoplasmic reticulum Ca2+ ATPase did not have an effect. The relaxation caused by the RRF was not altered by resveratrol incubation.
CONCLUSIONS
Cis- and trans-resveratrol relax bovine retinal arteries similarly and concentration dependently. The main relaxation mechanism remains unclear, but K+ channels, carbon monoxide, and the myosin phosphatase pathway may be involved. Resveratrol does not have an influence on the RRF.
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影响因子：
4.4
作者：
Sareen, Dhruv;van Ginkel, Paul R.;Polans, Arthur S.
通讯作者：
Polans, Arthur S.
影响因子：
3.9
作者：
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DOI：
10.1152/ajpheart.00418.2009
发表时间：
2010-03-01
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
Polans, Arthur S.
影响因子：
4.4
作者：
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通讯作者：
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