Layer-by-layer polyelectrolyte encapsulation of Mycoplasma pneumoniae for enhanced Raman detection.
Layer-by-layer polyelectrolyte encapsulation of Mycoplasma pneumoniae for enhanced Raman detection.
复制标题
肺炎支原体的层层聚电解质封装用于增强拉曼检测。
DOI：
10.1039/c4an00596a
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Dluhy,RichardA
中科院分区：
文献类型：
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作者：
Rivera-Betancourt,OmarE;Sheppard,EdwardS;Krause,DuncanC;Dluhy,RichardA
肺炎支原体是人类呼吸道疾病的主要原因，占所有社区获得性肺炎的20%。现有的支原体诊断主要受限于从临床样品培养细菌的不良成功率。迫切需要开发具有高灵敏度、特异性和便利性的用于支原体检测的新平台。在这里，我们报告的逐层（LBL）封装的M。肺炎细胞与Ag纳米颗粒在聚电解质聚（烯丙胺盐酸盐）（PAH）和聚（苯乙烯磺酸盐）（PSS）的基质中的细胞。我们评估了纳米颗粒包封的支原体细胞作为分化支原体的平台。pneumoniae菌株使用表面增强拉曼散射（Sers）结合多元统计分析。三个不同的M对M129、FH和II-3三株肺炎克雷伯氏菌进行了研究。扫描电子显微镜和荧光成像表明，银纳米粒子之间的相反电荷的双电层。Sers光谱表明，LBL封装提供了优异的光谱再现性。多元统计分析的拉曼光谱区分三个M。pneumoniae菌株，特异性和敏感性为97-100%，均方根误差低（0.1-0.4）。这些结果表明，纳米粒和微胶囊包封M。pneumoniae是一个潜在的强大平台，用于快速和灵敏的基于SERS的细菌鉴定。
Mycoplasma pneumoniae is a major cause of respiratory disease in humans and accounts for as much as 20% of all community-acquired pneumonia. Existing mycoplasma diagnosis is primarily limited by the poor success rate at culturing the bacteria from clinical samples. There is a critical need to develop a new platform for mycoplasma detection that has high sensitivity, specificity, and expediency. Here we report the layer-by-layer (LBL) encapsulation of M. pneumoniae cells with Ag nanoparticles in a matrix of the polyelectrolytes poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS). We evaluated nanoparticle encapsulated mycoplasma cells as a platform for the differentiation of M. pneumoniae strains using surface enhanced Raman scattering (SERS) combined with multivariate statistical analysis. Three separate M. pneumoniae strains (M129, FH and II-3) were studied. Scanning electron microscopy and fluorescence imaging showed that the Ag nanoparticles were incorporated between the oppositely charged polyelectrolyte layers. SERS spectra showed that LBL encapsulation provides excellent spectral reproducibility. Multivariate statistical analysis of the Raman spectra differentiated the three M. pneumoniae strains with 97–100% specificity and sensitivity, and low (0.1–0.4) root mean square error. These results indicated that nanoparticle and polyelectrolyte encapsulation of M. pneumoniae is a potentially powerful platform for rapid and sensitive SERS-based bacterial identification.