Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures
Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures
复制标题
DOI：
10.1016/j.cellbi.2006.01.010
复制
发表时间：
2006-06-01
影响因子：
3.9
通讯作者：
Kurihara, H
中科院分区：
文献类型：
--
作者：
Mizuno, N;Shiba, H;Kurihara, H
细胞治疗的主要问题是病毒或细菌感染和免疫反应的可能性。因此，预期使用自体血清而非常规牛血清重新植入培养细胞。用分化成各种细胞的人类间充质干细胞（hMSC）进行细胞疗法被认为是治愈性的。为了用人自体血清（human autologous serum，HAS）培养hMSC，并将其回植用于细胞治疗，我们建立了一种完全封闭的分离血清的袋式培养系统，比较了HAS和胎牛血清（fetal calf serum，FCS）培养hMSC的增殖和多能性。HAS是简单，安全，有效地获得与开发的封闭袋系统。HAS中培养的hMSC细胞增殖能力强于FCS。hMSC暴露于含有HAS以及FCS的确定的诱导培养基中，分化为成骨细胞和脂肪细胞。这些发现表明，用开发的封闭袋系统获得的HAS在降低病毒或细菌感染和外源蛋白污染的风险以及增强hMSC的增殖方面是有利的。(c)2006年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
The major problem in cell therapy is the possibility of viral or bacterial infection and immune reactions. Therefore, it is expected of culture cells which are intended to be re-implanted with autologous serum rather than conventional bovine serum. Cell therapy with human mesenchymal stem cells (hMSC), differentiating to various cells, is thought to be curative. To culture hMSC with human autologous serum (HAS) and reimplant them for cell therapy, we developed a completely closed bag system separating serum, comparing proliferation and multipotency of hMSC cultured in HAS with those in foetal calf serum (FCS). HAS was simply, safely and efficiently obtained with the developed closed bag system. Cell proliferation of hMSC cultured in HAS was greater than that in FCS. hMSC, exposed to the defined induction medium containing HAS as well as FCS, differentiated into osteoblasts and adipocytes. These findings suggest that HAS obtained with the developed closed bag system is advantageous in a point of decrease in risk of virus or bacterial infection and foreign protein contamination and enhancement of proliferation of hMSC. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.