THE EFFECT OF ATP, GTP AND CAMP ON THE CGMP-DEPENDENT CONDUCTANCE OF THE FRAGMENTS FROM FROG ROD PLASMA-MEMBRANE
THE EFFECT OF ATP, GTP AND CAMP ON THE CGMP-DEPENDENT CONDUCTANCE OF THE FRAGMENTS FROM FROG ROD PLASMA-MEMBRANE
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DOI：
10.1016/0014-5793(89)80218-2
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发表时间：
1989-03-13
期刊：
影响因子：
3.5
通讯作者：
FESENKO, EE
中科院分区：
文献类型：
--
作者：
FILATOV, GN;JAINAZAROV, AB;FESENKO, EE
使用“膜片钳”方法，在“由内而外”配置中，ATP、ADP、AMP‐PCP和AMP‐PNP已被证明可使视杆细胞质膜电导的cGMP依赖性成分增加2-4倍，GTP、GDP而非GMP或不可水解的GTP类似物GMP‐PNP和GTP‐γ‐S可消除ATP作用。当镁和钙离子不存在时，在[EDTA] = 1 mM时观察到ATP和GTP效应。在大约一半的实验中，cGMP依赖性电导被微摩尔浓度范围内的cAMP增加10- 50%，cAMP作用不依赖于核苷三磷酸的存在。体内ATP、GTP和cAMP被认为调节感光细胞质膜对cGMP的敏感性。
Using a ‘patch‐clamp’ method in the ‘inside‐out’ configuration, ATP, ADP, AMP‐PCP and AMP‐PNP have been shown to increase the cGMP‐dependent component of the rod plasma membrane conductance 2–4‐fold and GTP, GDP but not GMP or nonhydrolyzable GTP analogs GMP‐PNP and GTP‐γ‐S to abolish the ATP action. The ATP and GTP effects were observed at [EDTA] = 1 mM when magnesium and calcium ions were absent. In about half of the experiments the cGMP‐dependent conductance was shown to be increased by cAMP in the micromolar concentration range by 10–50%, the cAMP action did not depend on the presence of nucleoside triphosphates. In vivo ATP, GTP and cAMP are assumed to modulate the sensitivity of the photoreceptor plasma membrane to cGMP.