Dermatophyte Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Dermatophyte Identification Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
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DOI：
10.1128/jcm.01280-11
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发表时间：
2011-12-01
影响因子：
9.4
通讯作者：
Wengenack, Nancy L.
中科院分区：
文献类型：
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作者：
Theel, Elitza S.;Hall, Leslie;Wengenack, Nancy L.
比较了Bruker Biotyper基质辅助激光解吸电离飞行时间（MALDITOF）质谱仪（MS）鉴定临床培养物中皮肤真菌的性能与使用28 S rRNA基因测序鉴定皮肤真菌的性能。单独使用MALDI Biotyper文库（MBL;版本3.0），并与含有另外20种皮肤真菌谱（S-MBL）的补充文库组合使用。使用生物学家推荐的评分（属，>= 1.7;种，>= 2.0）和本研究建立的调整截止值（属，>= 1.5;种，> 1.7）解释获得的光谱;鉴定要求前两种不同生物之间的评分差异至少为10%，才被认为是正确的。使用两个库和两组截止标准评价了100个良好表征的存档皮肤真菌分离株和71个新鲜皮肤真菌培养物。总的来说，当使用调整后的评分标准时，S-MBL在属（93%对37.4%; P <0.0001）和种（59.6%对20.5%; P < 0.0001）水平上均显著优于MBL。重要的是，应用降低的截止值显著改善了S-MBL的属（P = 0.005）和种（P < 0.0001）水平的鉴定，而不会导致错误鉴定的增加。MALDI-TOF MS是传统或分子方法用于皮肤真菌鉴定的具有成本效益和快速的替代方法，前提是补充参考文库以充分涵盖临床相关的种内菌株多样性。
The performance of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDITOF) mass spectrometer (MS) for the identification of dermatophytes from clinical cultures was compared to that of dermatophyte identification using 28S rRNA gene sequencing. The MALDI Biotyper library (MBL; version 3.0) was used alone and in combination with a supplemented library containing an additional 20 dermatophyte spectra (S-MBL). Acquired spectra were interpreted using both the manufacturer-recommended scores (genus, >= 1.7; species, >= 2.0) and adjusted cutoff values established by this study (genus, >= 1.5; species, > 1.7); identifications required a minimum 10% difference in scores between the top two different organisms to be considered correct. One hundred well-characterized, archived dermatophyte isolates and 71 fresh dermatophyte cultures were evaluated using both libraries and both sets of cutoff criteria. Collectively, the S-MBL significantly outperformed the MBL at both the genus (93% versus 37.4%; P < 0,0001) and species (59.6% versus 20.5%; P < 0.0001) levels when using the adjusted score criteria. Importantly, application of the lowered cutoff values significantly improved genus (P = 0.005)- and species (P < 0.0001)-level identification for the S-MBL, without leading to an increase in misidentifications. MALDI-TOF MS is a cost-effective and rapid alternative to traditional or molecular methods for dermatophyte identification, provided that the reference library is supplemented to sufficiently encompass clinically relevant, intraspecies strain diversity.