Investigation of multipotent postnatal stem cells from human periodontal ligament
Investigation of multipotent postnatal stem cells from human periodontal ligament
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DOI：
10.1016/s0140-6736(04)16627-0
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发表时间：
2004-07-10
期刊：
影响因子：
168.9
通讯作者：
Shi, ST
中科院分区：
文献类型：
--
作者：
Seo, BM;Miura, M;Shi, ST
背景牙周病导致牙周组织（包括牙周韧带（PDL）、牙骨质和骨）的破坏，是成人牙齿缺失的主要原因，也是全球范围内的重大公共卫生负担。牙周韧带是一种特殊的结缔组织，连接牙骨质和牙槽骨，以维持和支持牙齿原位，并保持组织体内平衡。我们研究了人类牙周膜含有可用于再生牙周组织的干细胞的概念。19方法从25颗手术拔除的人第三磨牙中获取牙周膜组织，采用单克隆选择和磁激活细胞分选法分离牙周膜干细胞（PDLSCs）。免疫组织化学染色，RT-PCR，和北方和蛋白质印迹分析被用来确定假定的干细胞标志物。将人PDLSC移植到免疫功能低下的小鼠（n=12）和大鼠（n=6），以评估组织再生和牙周修复的能力。在确定的培养条件下，PDLSC分化为成牙骨质细胞样细胞、脂肪细胞和胶原形成细胞。当移植到免疫功能低下的啮齿类动物，PDLSCs显示出产生牙骨质/PDL样结构的能力，并有助于牙周组织repairment.Interpretation我们的研究结果表明，PDL含有干细胞，有潜力产生牙骨质/PDL样组织在体内。这些细胞的移植，可以从一个容易获得的组织资源和扩大离体，可能持有作为一种治疗方法重建牙周病破坏的组织的承诺。
Background Periodontal diseases that lead to the destruction of periodontal tissues-including periodontal ligament (PDL), cementum, and bone-are a major cause of tooth loss in adults and are a substantial public-health burden worldwide. PDL is a specialised connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homoeostasis. We investigated the notion that human PDL contains stem cells that could be used to regenerate periodontal tissue. 19Methods PDL tissue was obtained from 25 surgically extracted human third molars and used to isolate PDL stem cells (PDLSCs) by single-colony selection and magnetic activated cell sorting. Immunohistochemical staining, RT-PCR, and northern and western blot analyses were used to identify putative stem-cell markers. Human PDLSCs were transplanted into immunocompromised mice (n=12) and rats (n=6) to assess capacity for tissue regeneration and periodontal repair.Findings PDLSCs expressed the mesenchymal stem-cell markers STRO-1 and CD146/MUC18. Under defined culture conditions, PDLSCs differentiated into cementoblast-like cells, adipocytes, and collagen-forming cells. When transplanted into immunocompromised rodents, PDLSCs showed the capacity to generate a cementum/PDL-like structure and contribute to periodontal tissue repair.Interpretation Our findings suggest that PDL contains stem cells that have the potential to generate cementum/PDL-like tissue in vivo. Transplantation of these cells, which can be obtained from an easily accessible tissue resource and expanded ex vivo, might hold promise as a therapeutic approach for reconstruction of tissues destroyed by periodontal diseases.