Confocal SERS Mapping of Glycan Expression for the Identification of Cancerous Cells
Confocal SERS Mapping of Glycan Expression for the Identification of Cancerous Cells
复制标题
DOI：
10.1021/ac4038762
复制
发表时间：
2014-05-20
影响因子：
7.4
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
Craig, Derek;McAughtrie, Sarah;Graham, Duncan
凝集素功能化的银纳米颗粒已被成功地设计成用作分子成像剂，以研究哺乳动物细胞表面的碳水化合物凝集素相互作用，使用表面增强拉曼散射（Sers）。碳水化合物-凝集素相互作用是许多细胞过程的关键，并且负责控制一系列细胞相互作用。在这项研究中，凝集素功能化的银纳米粒子被用来检测在细胞界面的碳水化合物物种的表达。碳水化合物-凝集素相互作用使用三种不同的凝集素种类为三种不同的细胞类型。由于已知相同来源的癌细胞与非癌细胞中聚糖表达之间的差异，该方法已扩展到研究癌性和非癌性前列腺细胞。这已经通过共聚焦Sers映射这些细胞类型中的每一种的表面上的关键聚糖、唾液酸的表达来实现。在实现这样的歧视，一种新的方法已经创建，通过该方法可以重复监测聚糖的表达。使用荧光和Sers进行比较研究。当分析细胞亚群以区分癌细胞和非癌细胞时，Sers提供了对荧光的增加的区分。这种方法的成功意味着它可以用来补充目前的金标准组织病理学技术。
Lectin-functionalized silver nanoparticles have been successfully designed for use as molecular imaging agents to investigate carbohydrate lectin interactions at the surface of mammalian cells, using surface-enhanced Raman scattering (SERS). Carbohydrate-lectin interactions are key to many cellular processes and are responsible for controlling an array of cellular interactions. In this study, lectin-functionalized silver nanoparticles were used to detect the expression of carbohydrate species at the cellular interface. The carbohydrate-lectin interactions were demonstrated using three different lectin species for three distinct cell types. Due to the known difference between the expressions of glycans in cancerous versus noncancerous cells of the same origin, this approach has been expanded to study both cancerous and noncancerous prostate cells. This has been achieved via confocal SERS mapping of the expression of the key glycan, sialic acid, on the surface of each of these cell types. In achieving such discrimination, a novel method has been created by which glycan expression can be reproducibly monitored. Comparative studies were performed using both fluorescence and SERS. SERS provided an increased discrimination over fluorescence when analyzing cell subsets to discriminate between cancerous and noncancerous cells. The success of this method means that it could be used to complement the current gold standard histopathological techniques.