Point-of-care SARS-CoV-2 serological assays for enhanced case finding in a UK inpatient population.
Point-of-care SARS-CoV-2 serological assays for enhanced case finding in a UK inpatient population.
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DOI：
10.1038/s41598-021-85247-w
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发表时间：
2021-03-12
影响因子：
4.6
通讯作者：
Moore LSP
中科院分区：
文献类型：
--
作者：
Pallett SJC;Denny SJ;Patel A;Charani E;Mughal N;Stebbing J;Davies GW;Moore LSP
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. Case identification is currently made by real-time polymerase chain reaction (PCR) during the acute phase and largely restricted to healthcare laboratories. Serological assays are emerging but independent validation is urgently required to assess their utility. We evaluated five different point-of-care (POC) SARS-CoV-2 antibody test kits against PCR, finding concordance across the assays (n = 15). We subsequently tested 200 patients using the OrientGene COVID-19 IgG/IgM Rapid Test Cassette and find a sensitivity of 74% in the early infection period (day 5–9 post symptom onset), with 100% sensitivity not seen until day 13, demonstrating inferiority to PCR testing in the infectious period. Negative rate was 96%, but in validating the serological tests uncovered potential false-negatives from PCR testing late-presenting cases. A positive predictive value (PPV) of 37% in the general population precludes any use for general screening. Where a case definition is applied however, the PPV is substantially improved (95.4%), supporting use of serology testing in carefully targeted, high-risk populations. Larger studies in specific patient cohorts, including those with mild infection are urgently required to inform on the applicability of POC serological assays to help control the spread of SARS-CoV-2 and improve case finding of patients that may experience late complications.
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影响因子：
2
作者：
Zhao, Jijun;Wang, Qin;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
76.2
作者：
Pallett, Scott J. C.;Rayment, Michael;Davies, Gary W.
通讯作者：
Davies, Gary W.
影响因子：
19
作者：
Walker, N. F.;Brown, C. S.;Brooks, T.
通讯作者：
Brooks, T.
DOI：
10.12182/20200360505
发表时间：
2020-03-01
期刊：
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
影响因子：
--
作者：
Luan, Rong-Sheng;Wang, Xin;Sichuan University Covid-, Emergency Research Group
通讯作者：
Sichuan University Covid-, Emergency Research Group
影响因子：
64.8
作者：
Woelfel, Roman;Corman, Victor M.;Wendtner, Clemens
通讯作者：
Wendtner, Clemens