Parenteral Lipid Emulsion Induces Germination of Candida albicans and Increases Biofilm Formation on Medical Catheter Surfaces
Parenteral Lipid Emulsion Induces Germination of Candida albicans and Increases Biofilm Formation on Medical Catheter Surfaces
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DOI：
10.1086/600106
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发表时间：
2009-08-01
影响因子：
6.4
通讯作者：
Ghannoum, Mahmoud A.
中科院分区：
文献类型：
--
作者：
Swindell, Kim;Lattif, Ali Abdul;Ghannoum, Mahmoud A.
背景。通过导管给予患者肠外营养，包括脂质乳剂（LE），是导致念珠菌病发生的一个原因不明的危险因素。萌发和生物膜的形成是公认的白色念珠菌的毒力决定因素。目前还没有研究表明LE对念珠菌生物膜产生的影响。在本研究中，我们研究了LE对念珠菌萌发及其在医用导管材料上形成生物膜的能力的影响。白色念珠菌SC-5314菌株在有或没有市售LE的条件下在标准培养基中生长。在这些介质中生长在硅弹性体导管盘上的生物膜通过干重测量进行质量比较。采用扫描电镜和激光共聚焦显微镜对生物膜形态进行分析。通过显微镜观察和菌落形成单位测定，分别评价了LE对白色念珠菌萌发和生长的影响。在标准培养基中添加LE可提高白色念珠菌生物膜的产量，并可观察到生物膜形态和结构的变化。此外，LE还能诱导白色念珠菌萌发并支持其生长。LE诱导的念珠菌毒力决定因素，如萌发和增强的生物膜生成，可能有助于解释通过医疗导管接受LE的患者中念珠菌的风险增加。
Background. The administration of parenteral nutrition, including lipid emulsion ( LE), to patients via medical catheters is an unexplained risk factor for the development of candidemia. Germination and biofilm formation are recognized virulence determinants of Candida albicans. No studies have addressed the effect of LE on candidal biofilm production. In this study, we investigated the effect of LE on candidal germination and its ability to form biofilm on medical catheter material.Methods. C. albicans strain SC-5314 was grown in standard growth medium in the presence or absence a commercially available LE. Biofilms grown on silicone-elastomer catheter discs in these media were compared for mass by dry weight measurements. Biofilm morphology was analyzed by scanning electron microscopy and confocal laser microscopy. The effect of LE on C. albicans germination and growth was evaluated microscopically and by determination of colony-forming units, respectively.Results. Addition of LE to standard growth medium increased C. albicans biofilm production and resulted in observed changes in biofilm morphology and architecture. Furthermore, LE induced germination and supported the growth of C. albicans.Conclusions. LE-inducible candidal virulence determinants, such as germination and enhanced biofilm production, may help to explain the increased risk of candidemia in patients receiving LE via medical catheters.