MoS2-LA-PEI nanocomposite carrier for real-time imaging of ATP metabolism in glioma stem cells co-cultured with endothelial cells on a microfluidic system
MoS2-LA-PEI nanocomposite carrier for real-time imaging of ATP metabolism in glioma stem cells co-cultured with endothelial cells on a microfluidic system
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MoS2-LA-PEI纳米复合载体用于在微流体系统上与内皮细胞共培养的胶质瘤干细胞中实时成像ATP代谢
DOI：
10.1016/j.bios.2017.07.046
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发表时间：
2018-01-15
影响因子：
12.6
通讯作者：
Lin, Jin-Ming
中科院分区：
文献类型：
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作者：
Li, Nan;Zhang, Weifei;Lin, Jin-Ming
近年来，刺激响应型载体引起了广泛的关注。然而，由于在生物环境中的低效率、不稳定性和细胞毒性等限制，刺激响应载体的长期和实时跟踪能力仍处于婴儿阶段。在这项工作中，我们开发了一种还原敏感载体，该载体由硫辛酸修饰的低分子量聚乙烯亚胺（LA-PEI）和通过二硫键结合的大表面比MoS 2纳米片组成，以模拟高分子量PEI。荷正电的载体负载带负电荷的适体进入细胞，用于真实的长期跟踪胶质瘤干细胞（GSC）在由HUVEC分泌的TGF β因子刺激时的三磷酸腺苷（ATP）代谢。我们设想MoS 2-LA-PEI载体具有很好的潜力，用于以低细胞毒性和高效率递送和监测活细胞中的变化。
Stimuli-responsive carriers have extensively attracted attention in recent years. However, long-term and real-time tracking ability with stimuli-responsive carrier is still in its infant stage due to the limitations such as, low efficacy, instability and cytotoxicity in a bio-environment. In this work, we developed a reduction-sensitive carrier composed of lipoic acid-modified low molecular weight polyethyleneimine (LA-PEI) and large surface ratio MoS2 nanosheet integrated via disulfide bond to mimic a high molecular weight PEI. The positively charged carriers loading negatively charged aptamer enter the cells for a real time long-term tracking of adenosine triphosphate (ATP) metabolism in glioma stem cells (GSCs) when stimulated by TGF beta factor secreted from HUVECs. We envision that MoS2-LA-PEI carrier has a promising potential for delivery and monitoring the changes in live cells with low cytotoxicity and high efficiency.