A 27-band snapshot hyperspectral imaging system for label-free tumor detection during image-guided surgery
A 27-band snapshot hyperspectral imaging system for label-free tumor detection during image-guided surgery
复制标题
27 波段快照高光谱成像系统，用于图像引导手术期间的无标记肿瘤检测
DOI：
10.1117/12.2508944
复制
发表时间：
2019
期刊：
影响因子：
3.8
通讯作者：
V. Gruev
中科院分区：
文献类型：
--
作者：
S. Blair;Missael Garcia;Christian J. Konopka;L. Dobrucki;V. Gruev
虽然荧光图像引导手术通过允许在切除过程中识别肿瘤为癌症患者提供了改善的治疗结果，但由于荧光分子标记物的漫长且昂贵的批准过程，它一直受到转化为临床实践缓慢的困扰。图像引导手术的无标记方法通过基于肿瘤微环境和周围解剖结构之间的光谱反射率和自发荧光的差异来区分癌性和非癌性组织提供了一种替代方案。不幸的是，能够监测整个手术部位的光谱差异的最先进的高光谱成像系统利用复杂的光学机械架构，其导致低图像分辨率、低帧速率和无法校准的配准误差，使得这些仪器在要求苛刻的手术工作流程期间不切实际。为了提供无标签的手术指导，同时解决现有系统的局限性，我们开发了一种单芯片快照高光谱成像系统，可提供从~450 nm到~750 nm的27个光谱波段。通过单片集成堆叠的光电二极管图像传感器与像素化干涉滤波器，我们已经产生了一个高度紧凑的成像系统，以每秒17.2帧的速率实现了1252 × 852像素的分辨率，同时避免了配准误差。该系统提供约55 dB的信噪比和约62 dB的动态范围，并且可以在标准宽带手术光源下实现光谱辨别。在小鼠模型中的人前列腺肿瘤植入物的临床前图像已被检查和呈现，以证明成像系统可以区分癌性和非癌性组织，并可以区分不同的癌症类型。
While fluorescence image-guided surgery offers improved treatment outcomes for patients with cancer by permitting the identification of tumors during resection, it has been plagued by slow translation into clinical practice due to the lengthy and costly approval process for fluorescent molecular markers. Label-free approaches to image-guided surgery provide an alternative by discriminating between cancerous and noncancerous tissue based on differences in spectral reflectance and autofluorescence between the tumor microenvironment and the surrounding anatomy. Unfortunately, state-of-the-art hyperspectral imaging systems capable of monitoring spectral differences across the entire surgical site utilize complex optomechanical architectures that contribute to low image resolutions, low frame rates, and co-registration error that cannot be calibrated, making these instruments impractical during demanding surgical workflows. To provide label-free surgical guidance while addressing limitations with existing systems, we have developed a single-chip snapshot hyperspectral imaging system that provides 27 spectral bands from ~450 nm to ~750 nm. By monolithically integrating a stacked photodiode image sensor with pixelated interference filters, we have produced a highly compact imaging system that achieves a resolution of 1252-by-852 pixels at a rate of 17.2 frames per second while avoiding co-registration error. The system provides a signal-to-noise ratio of ~55 dB and a dynamic range of ~62 dB, and it can enable spectral discrimination under standard broadband surgical light sources. Preclinical images of human prostate tumor implants in a murine model have been examined and presented to demonstrate that the imaging system can differentiate between cancerous and noncancerous tissue and can discriminate between distinct cancer types.
DOI：
10.1016/j.physrep.2015.12.004
发表时间：
2016-02-29
期刊：
Physics reports
影响因子：
--
作者：
Gao L;Wang LV
通讯作者：
Wang LV