Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C
Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C
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DOI：
10.1053/gast.2003.50140
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发表时间：
2003-04-01
期刊：
影响因子：
29.4
通讯作者：
Mizokami, M
中科院分区：
文献类型：
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作者：
Sugauchi, F;Orito, E;Mizokami, M
背景和目的：已报道 B 基因型乙型肝炎病毒 (HBV/B) 分离株在前核心区和核心基因上与 C 基因型重组或未重组。方法：所有 41 株具有 C 基因型重组的 HBV/B 分离株 (HBV/Ba) 均具有 A 的核苷酸 1838，而所有 29 株未发生重组的 HBV/B 分离株 (HBV/Bj) 则具有 G 的核苷酸 1838。利用这种单核苷酸多态性，开发了一种区分 HBV/Ba 和 HBV/Bj 的限制性片段长度多态性方法。结果：来自日本的 97 名 HBV/B 携带者中，有 90 名 (93%) 检测到 HBV/Bj，而来自其他国家的所有 177 名 HBV/B 携带者中均检测到 HBV/Bj（中国，20 名；香港，45 名；台湾，32 名；泰国，30 名；越南，30 名；美国，20 名[均为亚洲族裔]）。在一项病例对照研究中，乙型肝炎 e 抗原 (HBeAg) 和核心启动子双突变 (T1762/A1764) 在 80 名 HBV/Ba 携带者中的出现频率显着高于 HBV/Bj（分别为 35% vs. 18%，P < 0.05 和 33% vs. 15%，P < 0.05）。 30岁以上的HBV/Bj和HBV/Ba携带者之间的HBeAg患病率差异更为显着（66人中的5人或8% vs 62人中的16人或26%，P < 0.01）。结论：除日本外，亚洲地区 HBV/B 与 C 基因型重组很常见，并且 HBV/Ba 携带者中 HBeAg 比 HBV/Bj 携带者更常见。
Background & Aims: Hepatitis B virus (HBV) isolates of genotype B (HBV/B) with or without the recombination with genotype C over the precore region plus core gene have been reported. Methods: All the 41 HBV/B isolates having the recombination with genotype C (HBV/Ba) possessed the nucleotide 1838 of A in contrast to that of G in all 29 of those without the recombination (HBV/ Bj). Taking advantage of this single nucleotide polymorphism, a restriction fragment length polymorphism method was developed that distinguished HBV/Ba from HBV/Bj. Results: HBV/Bj was detected in 90 of the 97 (93%) carriers of HBV/B from Japan, whereas HBV/Ba occurred in all 177 carriers of HBV/B from other countries (China, 20; Hong Kong, 45; Taiwan, 32; Thailand, 30; Vietnam, 30; and the United States, 20 [all of an Asian ethnicity]). In a case-control study, hepatitis B e antigen (HBeAg) and the double mutation in the core promoter (T1762/A1764) were significantly more frequent in 80 carriers each of HBV/Ba than HBV/Bj (35% vs. 18%, P < 0.05 and 33% vs. 15%, P < 0.05, respectively). Differences in the prevalence of HBeAg were more conspicuous between the carriers of HBV/Bj and HBV/Ba older than 30 years (5 of 66 or 8% vs. 16 of 62 or 26%, P < 0.01). Conclusions: HBV/B having the recombination with genotype C is frequent in Asia, except in Japan, and HBeAg is more prevalent in carriers of HBV/Ba than HBV/Bj.