ASCORBIC-ACID IN CEREBROSPINAL-FLUID - A POSSIBLE PROTECTION AGAINST FREE-RADICALS IN THE BRAIN
ASCORBIC-ACID IN CEREBROSPINAL-FLUID - A POSSIBLE PROTECTION AGAINST FREE-RADICALS IN THE BRAIN
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DOI：
10.1016/0167-4943(95)00654-4
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发表时间：
1995-07-01
影响因子：
4
通讯作者：
DEGRELL, I
中科院分区：
文献类型：
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作者：
BARABAS, J;NAGY, E;DEGRELL, I
抗坏血酸在生物体中的功能是复杂的。以前的研究强调它对自由基有害影响的保护作用，并且它的存在对于许多酶的功能是必需的，抗坏血酸是一种强大的还原剂，由于其二烯醇分子结构，其不存在于氧化形式脱氢抗坏血酸中。抗坏血酸和脱氢抗坏血酸的比值可能是氧化还原过程的一个标志。我们测定并比较了健康人和老年性痴呆患者血浆中抗坏血酸和脱氢抗坏血酸的水平。在老年痴呆症患者中，抗坏血酸和脱氢抗坏血酸水平也在脑脊液中进行了测量。通过高效液相色谱-电化学检测法测定浓度。在老年痴呆症患者的血浆中，发现非常低的抗坏血酸水平（约100 mg/kg）。健康对照组的30%）。在腰椎脑脊液中，抗坏血酸的浓度是血浆水平的2.7倍。静脉输注抗坏血酸后，测量到脑脊液中的浓度缓慢但显著增加。我们的研究结果支持抗坏血酸通过血液-CSF屏障的主动转运过程。抗坏血酸水平可能是反映中枢神经系统抗自由基保护作用的重要因素。
The function of ascorbic acid in living organisms is complex. Previous studies emphasize its protective role against harmful effect of free radicals, and its presence is necessary for the function of numerous enzymes, Ascorbic acid is a powerful reducing agent due to its dienol molecular structure, which is not present in the oxidized form, dehydroascorbic acid. The ratio of ascorbic acid and dehydroascorbic acid might be a marker of oxidative-reductive processes, We measured and compared the level of ascorbic acid and dehydroascorbic acid in the plasma of healthy persons and those of senile dementia patients, who represent pathological aging of the brain. In senile dementia patients, ascorbic acid and dehydroascorbic acid levels were also measured in the cerebrospinal fluid. Concentrations were determined by high performance liquid chromatography with electrochemical detection. In the plasma of senile dementia patients, very low ascorbic acid levels were found (ca. 30% of the healthy control). In lumbar cerebrospinal fluid, the concentration of ascorbic acid is 2.7 times higher compared to that of the plasma level, After intravenous infusion of ascorbic acid, a slow but marked increase of the concentration in the cerebrospinal fluid was measured. Our results support an active transport process for ascorbic acid through the blood-CSF barrier. Ascorbic acid level might be an important factor representing the protection of the central nervous system against free radicals.