A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact.
A Large-scale Survey of CRF55_01B from Men-Who-Have-Sex-with-Men in China: implying the Evolutionary History and Public Health Impact.
复制标题
中国男男性行为者 CRF55_01B 的大规模调查：暗示进化历史和公共健康影响。
DOI：
10.1038/srep18147
复制
发表时间：
2015-12-15
影响因子：
4.6
通讯作者：
Shang H
中科院分区：
文献类型：
--
作者：
Han X;Takebe Y;Zhang W;An M;Zhao B;Hu Q;Xu J;Wu H;Wu J;Lu L;Chen X;Liang S;Wang Z;Yan H;Fu J;Cai W;Zhuang M;Liao C;Shang H
HIV-1在男男性行为者（MSM）中的流行在中国继续扩大，涉及几种不同谱系的HIV-1毒株的共循环，包括亚型B和CRF01_AE。这种扩张为产生新的重组菌株创造了条件。2008 - 2013年对全国11个省市的MSM人群进行了分子流行病学调查。根据核苷酸序列分析，7省975例MSM中共检出19株CRF55_01B，阳性率为1.5% ~ 12.5%，占1.95%。从6例流行病学上未关联的MSM中扩增出近全长基因组（NFLG）序列用于分析进化历史，鉴定出由CRF01_AE和B亚型组成的相同基因组结构，在pol区有4个独特的重组断点。对CRF01_AE和B片段的贝叶斯分子钟分析表明，CRF55_01B最近的共同祖先时间估计在2000年左右。我们的研究发现，CRF55_01B已经在大多数HIV-1高发省份传播，这突出了持续监测HIV-1毒株动态变化、新重组病毒出现的重要性，以及实施针对中国MSM人群的有效预防措施的必要性。
The HIV-1 epidemic among men-who-have-sex-with-men (MSM) continues to expand in China, involving the co-circulation of several different lineages of HIV-1 strains, including subtype B and CRF01_AE. This expansion has created conditions that facilitate the generation of new recombinant strains. A molecular epidemiologic survey among MSM in 11 provinces/cities around China was conducted from 2008 to 2013. Based on pol nucleotide sequences, a total of 19 strains (1.95%) belonged to the CRF55_01B were identified from 975 MSM in 7 provinces, with the prevalence range from 1.5% to 12.5%. Near full length genome (NFLG) sequences from six epidemiologically-unlinked MSM were amplified for analyzing evolutionary history, an identical genome structure composed of CRF01_AE and subtype B with four unique recombination breakpoints in the pol region were identified. Bayesian molecular clock analyses for both CRF01_AE and B segments indicated that the estimated time of the most recent common ancestors of CRF55_01B was around the year 2000. Our study found CRF55_01B has spread throughout the most provinces with high HIV-1 prevalence and highlights the importance of continual surveillance of dynamic changes in HIV-1 strains, the emergence of new recombinants, and the need for implementing effective prevention measures specifically targeting the MSM population in China.