Spermidine synthase is prominently expressed in the striatal patch compartment and in putative interneurones of the matrix compartment
Spermidine synthase is prominently expressed in the striatal patch compartment and in putative interneurones of the matrix compartment
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DOI：
10.1111/j.1471-4159.2006.03721.x
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发表时间：
2006-04-01
影响因子：
4.7
通讯作者：
Laube, G
中科院分区：
文献类型：
--
作者：
Krauss, M;Langnaese, K;Laube, G
普遍存在的多胺、亚精胺和精胺被认为是谷氨酸受体和内向整流钾通道的调节剂。它们是由一组特定的酶合成的，其中亚精胺合酶是催化腐胺形成精胺前体亚精胺的限速步骤。亚精胺和精胺以前定位于星形胶质细胞，可能反映存储，而不是在这些细胞的合成。为了鉴定精脒和精胺在脑中合成的细胞来源，针对重组小鼠精脒合酶产生抗体。正如预期的那样，在已知表达高水平亚精胺和精胺的区域，如下丘脑室旁核和视上核，获得了强的亚精胺脱氢酶样免疫反应性。在纹状体中，精脒合酶被发现在神经元和神经元的补丁隔室（纹状体）的μ阿片受体的表达定义。然而，精脒合酶的独特表达模式仅与精脒和精胺产物在纹状体中的分布部分重叠。此外，亚精胺脱氢酶样免疫反应被认为是在补丁隔室贴壁假定的中间神经元。这些亚精胺脱氢酶阳性神经元不表达任何标记特征的主要纹状体interneurone类。在补丁室和相邻的假定interneurones的神经标记可能表明多胺在纹状体的房室间信号传导的作用。
The ubiquitous polyamines spermidine and spermine are known as modulators of glutamate receptors and inwardly rectifying potassium channels. They are synthesized by a set of specific enzymes in which spermidine synthase is the rate-limiting step catalysing the formation of the spermine precursor spermidine from putrescine. Spermidine and spermine were previously localized to astrocytes, probably reflecting storage rather than synthesis in these cells. In order to identify the cellular origin of spermidine and spermine synthesis in the brain, antibodies were raised against recombinant mouse spermidine synthase. As expected, strong spermidine synthase-like immunoreactivity was obtained in regions known to express high levels of spermidine and spermine, such as the hypothalamic paraventricular and supraoptic nuclei. In the striatum, spermidine synthase was found in neurones and the neuropil of the patch compartment (striosome) as defined by expression of the mu opiate receptor. The distinct expression pattern of spermidine synthase, however, only partially overlapped with the distribution of the products spermidine and spermine in the striatum. In addition, spermidine synthase-like immunoreactivity was seen in patch compartment-apposed putative interneurones. These spermidine synthase-positive neurones did not express any marker characteristic of the major striatal interneurone classes. The neuropil labelling in the patch compartment and in adjacent putative interneurones may indicate a role for polyamines in intercompartmental signalling in the striatum.